三聚氰胺对SD孕鼠及胎鼠的毒作用  

The Toxic Effects of Melamine on Pregnant SD Rats and Fetuses

作　　者：周建瑜[1] 刘秋妍[1] 林居纯[1] 何雪梅[1] 何丽霞[1] 王爽[1] 冯彩风[1] 胡艺彪 

机构地区：[1]四川农业大学动物医学院,四川雅安625014

出　　处：《四川动物》2014年第2期195-200,共6页Sichuan Journal of Zoology

基　　金：2011年四川农业大学年本科生科研兴趣培养计划(2011039)

摘　　要：为阐明三聚氰胺对孕鼠和胎鼠毒性作用，将40只SD孕鼠随机分为高剂量组（Ⅰ）、中剂量组（Ⅱ）、低剂量组（Ⅲ）及空白对照组（Ⅳ），共4组，于妊娠第6~19 天，分别经口灌服800 mg/kg.d、200 mg/kg.d、20 mg/kg.d和0 mg/kg.d三聚氰胺混悬液。染毒期间，观察孕鼠中毒表现，以及不同妊娠时间体重变化；于妊娠第20 天处死母鼠，检测血清尿素氮、肌酐及尿酸，胎鼠及胎盘重量等指标；观察母鼠和胎儿肾脏、胎盘组织病理变化。结果显示，在染毒期，Ⅰ、Ⅱ组母鼠出现精神差等症状；与Ⅳ组相比，Ⅰ、Ⅱ组母鼠、胎鼠和胎盘重量，血液生化指标有显著或极显著差异（P＜0.05或P＜0.01），且Ⅰ、Ⅱ组母鼠、胎鼠肾脏以及胎盘有明显病理变化；但3个剂量水平的三聚氰胺对胚胎的存活无影响，也不产生致畸作用。To investigate the toxic effect of melamine on pregnant rats and the fetuses, 40 pregnant SD rats from sixth to nineteenth day of pregnancy were randomly divided into four groups including high dose group （Ⅰ）, medium dose group （Ⅱ）, low dose group （Ⅲ） and control group （Ⅳ）. Each group was orally administered with different dosages of melamine （800 mg/kg.d, 200 mg/kg.d, 20 mg/kg.d and 0 mg/kg.d）, respectively. The symptoms of poisoning and the weight change during exposure of pregnant SD rats were observed. Rats were sacrificed in the twentieth day of pregnancy, and the levels of serum BUN, UA and CREA were tested. The histopathological changes of maternal and fetal kidney as well as placenta were observed. The results were shown as follows： the pregnant rats of groupⅠand Ⅱ had some toxic symptoms such as bad spirit; the weight and serum biochemical index parameters of groupⅠ and Ⅱwere significantly different （P〈0.01 or P〈0.05） with group Ⅳ, and there were significant pathological changes in the kidneys and placenta of group Ⅰand Ⅱ. However, three dosage levels of melamine had no effect on the survival of embryos and teratogenic effects.

关 键 词：三聚氰胺 SD大鼠 胚胎 毒性 

分 类 号：S859.82[农业科学—临床兽医学]