芫根总皂苷对饮食诱导肥胖大鼠减肥降脂机制研究  

Anti-obesity and Hypolipidemic Mechanism of Total Saponins from Brassica rapa L. in High-fat Diet Induced Obese Rats

作　　者：陈志鹃[1] 蒋思萍 冯成[1] 吴亚辉[1] 高平[1] 

机构地区：[1]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610065 [2]西藏自治区高原生物研究所,拉萨850001

出　　处：《四川动物》2014年第2期279-282,共4页Sichuan Journal of Zoology

摘　　要：以高脂饮食诱导建立肥胖大鼠模型,研究芫根总皂苷减肥降脂的作用机制。以奥利司他(48 mg/kg)为阳性药,设芫根总皂苷3个剂量组(500 mg/kg、1000 mg/kg、1500 mg/kg),灌胃给药45 d。检测血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、脂蛋白脂酶(LPL)、肝脂酶(HL)、卵磷脂胆固醇酰基转移酶(LCAT)、胰脂肪酶(PL)的活性及丙二醛(MDA)、游离脂肪酸(FFA)、瘦素(LEP)的含量。结果显示与高脂模型组相比,芫根总皂苷可不同程度升高SOD、GSH-Px、LPL、HL活性,抑制PL活性,降低MDA、FFA、LEP含量,其可能的作用机制是提高抗氧化能力,降低肝脂质损伤,促进脂质分解,抑制脂肪吸收,改善脂质代谢及瘦素抵抗等。To investigate the potential anti-obesity and hypolipidemic effects of total saponins from Brassica rapa L., the high-fat diet （HFD） induced obese rats model was constructed, and rats feed with orlistat were used as positive control. The obese rats of experimental groups were given three dosages （500 mg/kg, 1000 mg/kg, 1500 mg/kg） of total saponins from Brassica rapa L. for 45 days. Then the sera were collected to determine the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, lipoprotein lipase （LPL）, hepatic lipase （HL）, lecithin cholesterol acyltransferase （LCAT） and pancreatic lipase （PL）. The contents of malondialdehyde （MDA）, free fatty acid （FFA） and leptin （LEP） were also analyzed. Compared with the control group, the activities of SOD, GSH-Px, LPL and HL were increased in experimental groups. On the other hand, the MDA and leptin levels of sera were significantly impaired and the PL activity was inhibited. These suggested that the anti-obesity and hypolipidemic effects of total saponins from Brassica rapa L. was probably antioxidation, anti-lipidperoxidation, which could promote lipolysis, inhibit fat absorption, improve lipid metabolism and leptin resistance.

关 键 词：芫根 总皂苷 肥胖 脂质代谢 作用机制 

分 类 号：R285.5[医药卫生—中药学]