水稻培矮64S空间诱变突变株系的花培效应研究  

Study on Anther Culture Effects of Mutant Lines of ‘Peiai 64S'

作　　者：黄翠红[1] 刘永柱[1] 陈立凯[1] 郭涛[1] 杨瑰丽[1] 陈志强[1] 王慧[1] 

机构地区：[1]华南农业大学国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《核农学报》2014年第3期386-392,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家"863"计划(2012AA101201);现代农业产业技术体系建设专项资金(CARS-01-12);广东省农业科技创新团队项目(2011A020102004)

摘　　要：利用"实践八号"农业卫星搭载籼稻光温敏不育系培矮64S的干种子,对诱变后代19个突变株系进行了花药培养研究,考察了愈伤组织的诱导与分化情况。结果表明,与对照原种相比空间诱变后代的花药培养力发生了变化,其中H17、H11、H14、H6花药培养力提高,突变后更适合于花药培养;另外,在改良白苗分化方面也有所成效,但效果不大,H8、H6的白苗分化率与对照原种相比得到了有效的降低,其中以H6综合性状表现较好。利用航天技术与花药培养技术相结合,不仅能快速筛选出花药培养力高的优良单株,同时为今后开展籼稻材料的花药培养和选育新的不育系研究打下基础。Dried seeds of indica rice Photoperiod-and thermo-sensitive genic male sterility（PGMS） Line Peiai64S were carried into space by satellite ‘Shijian No. 8’ and the mutagenic effects of space condition on the induction and differentiation of the callus of 19 mutant lines were studied. The anther culture ability of ‘Peiai 64S’ displayed a wide range of variation after space mutation, and the mutants H17,H11,H14 and H6 as PGMS line were screen out as suitable lines for anther culture. Moreover, the albino plant differentiation frequency of H8 and H6 was lower than CK, and H6 performed better in the aspects of the comprehensive characters. The combination of space conditions and anther culture could not only produce promising mutants with high anther culture ability,but also provide a foundation for the study of indica＇s anther culture and breeding of male sterile line.

关 键 词：培矮64S 诱变效应 花药培养 优良株系 

分 类 号：S511[农业科学—作物学]