源于大麦小孢子诱变-氮胁迫培养的DH株系田间耐低氮性表现  

Field Response to Low Nitrogen of Barley DH Lines from Microspores Mutagenesis and Culture under Nitrogen Stress

作　　者：陆瑞菊[1,2] 刘成洪[1,2] 何婷[1,2] 陈志伟[1,2] 徐红卫[1,2] 杜志钊[1,2] 高润红[1,2] 王亦菲[1,2] 邹磊[1,2] 郭桂梅[1,2] 卜姝明[3] 黄亦辰[3] 黄剑华[1,2] 

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《核农学报》2014年第3期412-417,共6页Journal of Nuclear Agricultural Sciences

基　　金：大麦青稞产业技术体系(CARS-05);上海市种业发展项目[沪农科种字(2012)第7号;上海市科委基础重点项目(12JC1407800)

摘　　要：以EMS处理大麦品种花30的游离小孢子,结合低氮胁迫培养获得再生植株,其自交一代的加倍单倍体(DH)株系,在正常施肥和低氮胁迫两种施肥条件下对DH株系进行鉴定,选择株高、单株有效穗和单株产量3个农艺性状作为鉴定指标,并与原始亲本花30进行比较。结果表明:在大田正常施肥和低氮胁迫条件下,DH株系中株高、有效穗和单株产量3个性状出现了明显的正向变异,在低氮胁迫下的表现更为明显。结论:利用游离小孢子的EMS处理结合低氮胁迫筛选培养,可以获取耐低氮性提高的纯合变异体。The isolated microspores from barley cultivar‘Hua 30’were treated with ethyl methane sulfonate （EMS）,and cultured under low nitrogen stress to generate plantlets. Their double haploid （DH） lines were planted in field under normal fertilization and low nitrogen stress to identify the low nitrogen tolerance mutants. Three agronomy traits including plant height, number of productive tillers per plant, and yield per plant were selected for evaluation against their parent of barley cv‘Hua 30’. The results showed that the positive variances of three traits were observed in DH lines comparing to their parents, under the normal fertilization and low nitrogen stress, especially in the latter condition. It is practicable to obtain the homozygous mutants with improved low nitrogen tolerance through microspores mutagenesis with EMS followed by culture under low nitrogen stress.

关 键 词：大麦 EMS诱变 低氮胁迫 小孢子培养 单株产量 

分 类 号：S512.3[农业科学—作物学]