快中子辐射对蝴蝶兰原球茎和茎段增殖分化的影响  

Effect of Fast Neutron Radiation on the Proliferation and Differentiation of Protocorm-like Bodies and Seedlings Stem Segments of Phalaenopsis

作　　者：张银洁[1] 李杰[1] 王芝娜[1] 郑春[2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]中国工程物理研究院核物理与化学研究所,四川绵阳621000

出　　处：《核农学报》2014年第3期440-445,共6页Journal of Nuclear Agricultural Sciences

基　　金：四川省教育厅资助科研项目(10ZA018);四川省科学技术带头人培养项目(11sd3105);西南科技大学科研基金(.09zx7107;.12xjjx90)

摘　　要：为了获得蝴蝶兰新种质以及开辟新的蝴蝶兰育种方法,利用快中子脉冲堆对蝴蝶兰不同品种原球茎和幼苗茎段进行辐照处理,结果表明:通过对处理材料的回归分析得到,火鸟原球茎的半致死注量为3279×108·cm-2,内山姑娘原球茎的半致死注量为4063×108·cm-2,火鸟茎段的半致死注量为2239×108·cm-2,内山姑娘茎段的半致死注量为2298×108·cm-2。辐射敏感性表现为火鸟>内山姑娘,幼苗茎段>原球茎。对于原球茎,辐照注量小于600×108·cm-2,对其增殖和分化有一定的促进作用;对于茎段,辐照注量小于600×108·cm-2,对其所形成的幼苗的生根没有影响,但小于300×108·cm-2,对所形成幼苗的增殖有促进作用。高注量(>2500×108·cm-2)辐照对原球茎的增殖和分化以及对茎段所形成的幼苗的增殖和生根均有显著的抑制作用。Different varieties of the Protocorm-like bodies （PLBs） and seedlings stem segments of phalaenopsis were irradiated with fast neutron impulse pile, the results showed that： Semi-lethal pouring dose（LD50） of neutron radiation on the PLBs of Firebird and Neyshan guniang were 3279×108·cm-2 and 4063×108·cm-2,on the Seedlings stem segments of Firebird and Neyshan guniang were 2239×108·cm-2 and 2298×108·cm-2, by the regression analysis of the processed material.Radiation sensitivity of phalaenopsis were Firebird 〉 Neyshan guniang and seedling stem segments 〉PLBs. There was a certain role in promoting the multiplication and differentiation of PLBs when the pouring dose of neutron radiation less than 600×108·cm-2. When the pouring dose of neutron radiation less than 600×108·cm-2, it was no impact on rooting of stem segments seedlings.When the pouring dose of neutron radiation less than 300×108·cm-2,neutron radiation had a role in promoting the multiplication of the seedlings stem segments.On the proliferation and differentiation of Protocorm-like bodies （PLBs） and the multiplication and rooting of stem segments seedlings, high fluence （〉2500×108·cm-2） irradiation showed a significant inhibition.

关 键 词：快中子辐射 蝴蝶兰 原球茎 幼苗茎段 

分 类 号：S682.31[农业科学—观赏园艺]