镉处理下增施氮对杨树叶绿素合成和叶绿体超微结构的影响  

Effects of Nitrogen Supplement on Chlorophyll Synthesis and Chloroplast Ultrastructure of Poplar Plants Under Cadmium Stress

作　　者：张帆[1] 万雪琴[2] 翟晶[1] 

机构地区：[1]四川农业大学风景园林学院,四川温江611130 [2]四川农业大学林学院,四川雅安625014

出　　处：《核农学报》2014年第3期485-491,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(31300514);四川省"十二五"科技攻关项目林木新品种选育(2011YZGG)

摘　　要：为进一步验证氮在植物解除重金属毒害中的积极作用和生理机制,本试验对杨树幼苗在镉胁迫下增施氮肥后的生长、叶绿素合成前体物的变化和叶绿体超微结构进行研究。结果表明:单纯的Cd处理显著抑制杨树植株的生长,破坏叶绿体结构,阻止叶绿素的合成,其受阻位点可能位于Urogen III到CoprogenIII之间;而在有效氮供应充足条件下,高浓度Cd处理的植株长势明显优于对照和单纯重金属处理的植株,而且叶绿素合成途径畅通,叶绿体结构完整。由此说明,氮在植物对重金属的解毒中起到重要作用,增施氮肥可缓解重金属对植物的毒害。In order to clarify the important physiological effect of nitrogen on plant exposed to heavy metals, poplar plants were used as experimental material and treated with CdCl2 plus NH4HCO3,CdCl2 and NH4HCO3, respectively. After 60 days of treatment, plant growth, relative content of precursor of chlorophyll synthesis and chloroplast ultrastructure were detected. Results showed that plant height, dry weight and chlorophyll content of the plants exposed to Cd all significantly decreased when compared to the controls. Cd seriously destroyed chloroplast ultrastructure and inhibited Chl synthesis. The blocking site located on the steps between Urogen Ⅲ and Coprogen Ⅲ; nitrogen addition could promote the growth, increase content of chlorophyll of plants under stress of Cd, and well protect the chloroplast ultrastructure, effectively alleviate the inhibition of Cd to chlorophyll synthesis by unblocking the pathway. The above results demonstrated that nitrogen played an important role in detoxifying heavy metals from plants.

关 键 词：杨树 镉 氮 叶绿素合成 超微结构 解毒作用 

分 类 号：S792.11[农业科学—林木遗传育种]