以高盐渗滤液富集筛选氨氧化菌试验  

Research on Enrichment Culture and Screening of Nitrosobacteria Strains Using Landfill Leachate with High Salinity

作　　者：傅金祥[1] 何祥[1] 贺一达 张荣新[1] 

机构地区：[1]沈阳建筑大学市政与环境工程学院,辽宁沈阳110168

出　　处：《沈阳建筑大学学报（自然科学版）》2014年第2期345-351,共7页Journal of Shenyang Jianzhu University：Natural Science

基　　金：国家水体污染控制与治理科技重大专项(2012ZX07212-001);辽宁环境科研教育"123工程"

摘　　要：目的研究利用渗滤液作为氨氧化菌富集培养基的可行性,筛选出的优势菌在不同条件下的脱氮效果,为渗滤液生物处理提供参考.方法通过对渗滤液富集后污泥进行氨氧化菌初筛和复筛结果选出的优势菌在静态试验下研究pH、进水底物浓度、溶解氧和盐度的影响和投加优势菌后氨氮氧化速率.结果 pH、溶解氧、底物质量浓度和盐度对优势菌的脱氮性能有一定的影响,优势菌的最佳pH为7.5~8,溶解氧质量浓度3 mg/L,进水底物质量浓度与所需溶解氧成正相关关系;优势菌表现出不同盐度耐受性,X1表现出耐盐特性,而X4表现出嗜盐特性.结论渗滤液高氨氮的特性使其有作为氨氧化菌富集培养基的条件,渗滤液富集筛选出的优势菌投加反应器后可有效提高反应器的氨氮去除率.This paper studies to use the feasibility of leachate as ammonia oxidizing bacteria broth,screen out the advantage of the denitrification effect under different conditions,provide reference for biological treatment of leachate. Of leachate concentration after the sludge at the beginning of ammonia oxidizing bacteria screening and screen out the advantage of bacteria under the static experimental study pH,concentration in w ater,dissolved oxygen,and the effect of salinity and ammonia oxidation rate after adding the advantage bacterium. Results show that the optimum pH advantage bacterium is 7. 5- 8,3 mg / L,dissolved oxygen concentration in the w ater concentration are positively correlated w ith the dissolved oxygen（ do） required, dominant fungi show different salinity tolerance,X1show s the salt-tolerant characteristics,and X4show s halophilic characteristics. It is concluded that the characteristics of leachate high ammonia nitrogen make it as a condition of ammonia oxidizing bacteria enrichment medium,pH,dissolved oxygen,substrate concentration and salinity on denitrification performance advantage bacteria have a certain influence,enrichment of dominant fungi by leachate dosing reactor can effectively improve the reactor after the conclusion of the ammonia nitrogen removal rate.

关 键 词：垃圾渗滤液 氨氧化菌 富集 盐质量浓度 

分 类 号：X703.1[环境科学与工程—环境工程]