切口痛-瑞芬太尼痛觉过敏大鼠脊髓和背根神经节δ阿片受体表达水平的变化  

Changes in δ opioid receptor expression in spinal cord and dorsal root ganglias in rats with hyperalgesia induced by incisional pain-remifentanil

作　　者：王春艳[1] 李依泽[1] 谢克亮[1] 王海云[1] 张麟临 舒瑞辰 于泳浩[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科天津市麻醉学研究所,300052

出　　处：《中华麻醉学杂志》2014年第1期29-32,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30972847,81371245）;天津市科技支撑项目（12ZCZDSY03000）

摘　　要：目的 探讨切口痛-瑞芬太尼痛觉过敏大鼠脊髓和背根神经节δ阿片受体表达水平的变化.方法 取尾静脉置管成功的成年雄性SD大鼠32只,体重260～ 280 g,采用随机数字表法将其分为4组（n=8）：对照组（C组）静脉输注生理盐水0.1 ml·kg-1 ·min-1 60 min;切口痛组（Ⅰ组）静脉输注生理盐水0.1 ml·kg-1 ·min-1 60 min,于输注即刻建立切口痛模型；瑞芬太尼组（R组）静脉输注瑞芬太尼1.0 μg· kg-1·min-1 60 min；瑞芬太尼＋切口痛组（RI组）静脉输注瑞芬太尼1.0 μg·kg-1 ·min-1 60min,于输注即刻建立切口痛模型.于静脉输注生理盐水或瑞芬太尼前24 h、输注后2、6、24和48 h（T0-4）时,在室温（18～22℃）安静环境下测定机械刺激缩足阈（PWT）和热刺激缩足潜伏期（PWL）.行为学测试结束后处死大鼠,取脊髓L4-6节段和背根神经节,采用Western blot法测定脊髓和背根神经节总蛋白及膜蛋白δ阿片受体的表达,并计算膜蛋白与总蛋白δ阿片受体表达的比值（m/t比值）.结果 与C组比较,Ⅰ组、R组和RI组T1-4时PWT降低,PWL缩短,总蛋白和膜蛋白δ阿片受体表达上调,m/t比值增加（P＜0.05或0.01）；与Ⅰ组比较,R组T1-4时PWT、PWL、总蛋白和膜蛋白δ阿片受体表达和m/t比值差异无统计学意义（P＞0.05）,RI组T1-4时PWT降低,PWL缩短,总蛋白和膜蛋白δ阿片受体表达上调,m/t比值增加（P＜0.05或0.01）；与R组比较,RI组T1-4时PWT降低,PWL缩短,总蛋白和膜蛋白δ阿片受体表达上调,m/t比值增加（P＜0.05或0.01）.结论 大鼠切口痛-瑞芬太尼痛觉过敏的形成可能与脊髓和背根神经节δ阿片受体表达上调和向细胞膜上转运增强有关.Objective To investigate the changes in δ opioid receptor expression in the spinal cord and dorsal root ganglias in rats with hyperalgesia induced by incisional pain （IP）-remifentanil.Methods Thirty-two adult male Sprague-Dawley rats in which the catheter was successfully placed in the caudal vein were randomly divided into 4 groups （n =8 each）：control gourp （group C）,IP group （group I）,remifentanil group （group R）,and remifentanil ＋ IP group （group RI）.Normal saline 0.1 ml· kg-1 · min-1 was infused intravenously for 60 min in group C.In group I,normal saline 0.1 ml · kg-1 · min-1 was infused intravenously for 60 min,and IP was induced immediately after onset of infusion.In group R,remifentanil 1.0 μg· kg-1 · min-1 was infused intravenously for 60 min.In group RI,remifentanil 1.0μg· kg-1 · min-1 was infused intravenously for 60 min and IP was induced immediately after onset of infusion.At 24 h before infusion and 2,6,24 and 48 h after infusion （T0-4）,paw withdrawal threshold （PWT） and paw withdrawal latency （PWL） were measured at room temperature （18-22 ℃）.The rats were sacrificed after the end of the behavioral test and L4-6 segments of the spinal cord and dorsal root ganglias were removed for determination of the expression of total protein and membrane protein δ opioid receptor by Western blot.The ratio of δ opioid receptors in the membrane protein to those in the total protein （m/t ratio） was calculated.Results Compared with group C,PWT was significantly decreased and PWL was shortenedat T1-4,and the expression of total prtein and membrance protein δ opioid receptor was up-regulated and m/t ratio was increased in I,R and RI groups （P 〈 0.05 or 0.01）.Compared with group I,no significant change was found in PWT and PWL at T1-4,and the expression of total prtein and membrance protein δ opioid receptor and m/ t ratio in R group （P 〉 0.05）,and PWT was significantly decreased and PWL was shortened at T1-4 and the expression of total prtein

关 键 词：疼痛 手术后 哌啶类 痛觉过敏 受体 阿片样 δ 脊髓 神经节 脊 

分 类 号：R614[医药卫生—麻醉学]