抗缪勒管激素对预测牛超数排卵效果研究初探  

Preliminary Study of Detecting Anti-Mullerian Hormone to Predict Superovulation Effect in Cattle

作　　者：毕江华[1,2] 冯春涛[1] 陈龙 余文莉[1,2] 

机构地区：[1]北京安伯胚胎生物技术中心,北京100107 [2]国家肉牛牦牛产业技术体系石家庄综合试验站,石家庄050051 [3]石家庄天泉良种奶牛有限公司,石家庄050051

出　　处：《中国奶牛》2014年第6期16-19,共4页China Dairy Cattle

基　　金：现代农业产业技术体系建设专项资金(nycytx-38);石家庄市国际科技合作课题(12750066A);国家科技支撑计划课题"家畜胚胎保存与性控双犊诱导技术研究与示范"(2011BAD19B01)

摘　　要：为探讨抗缪勒管激素（AMH）在预测牛超数排卵中的诊断价值，本试验用酶联免疫吸附法（ELISA）测定了13头和牛的血清AMH浓度，并与三次重复超排效果进行相关性分析。结果显示，AMH浓度和超排头均回收胚数、可用胚数呈正相关（r=0．53、0．54，P=0．07）；三次重复超排头均回收胚胎数5．1枚，头均可用胚胎2．9枚组，AMH浓度在500-800pg／mL；三次重复超排头均回收胚数11．5枚，可用胚胎6．1枚组，AMH浓度在800-1000pg／mL；三次重复超排头均回收胚数12．2枚，可用胚胎8．3枚组，AMH浓度在1000-1100pg／mL。可以得出，牛血清AMH浓度有预测牛超数排卵效果和卵巢储备功能的价值。The objective of this study was to investigate the anti-Mullerian hormone （AMH） diagnostic value in predicting superovulation in cattle. The levels of AMH concentration for 13 head of wagyu serum samples were detected with enzyme-linked immunosorbent assay （ELISA）, and correlation analysis with three times repeated superovulation of 13 head of wagyu was estimated. The results showed that AMH concentration was positively correlated with the mean of recovered embryos and the mean of available embryos obtained by three times repeated superovulation （r-0.53,0.54, P =0.07）. The group for the mean of recovered embryos was 5.1, the mean of available embryos was 2.9, AMH concentration was 500-800pg/mL The group of mean of recovered embryos was 11.5, the mean of available embryos was 6.1, AMH concentration was 800-1000pg/mL The group for the mean of recovered embryos was 12.2, the mean of available embryos was 8.3, AMH concentration was 1000-1100pg/mU. It could be drawn the conclusion that the detection of serum levels of AMH was sensitive in predicting superovulation effect and the ovarian reserve function.

关 键 词：牛 抗缪勒管激素 超排效果 

分 类 号：S823.3[农业科学—畜牧学]