Performance and microbial diversity of an expanded granular sludge bed reactor for high sulfate and nitrate waste brine treatment  

作　　者：Runhua Liao Yan Li Xuemin Yu Peng Shi Zhu Wang Ke Shen Qianqian Shi Yu Miao Wentao Li Aimin Li 

机构地区：[1]State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University [2]School of Materials Science and Engineering,Jingdezhen Ceramic Institute

出　　处：《Journal of Environmental Sciences》2014年第4期717-725,共9页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.51178215,51378251);the Jiangsu Provincial Natural Science Foundation(No.BK2011032,BK20130575);the Jiangxi Provincial Natural Science Foundation(No.2010GQC0106);the State Key Laboratory of Pollution Control and Resource Reuse Foundation(No.PCRRF12006)

摘　　要：The disposal of waste brines has become a major challenge that hinders the wide application of ion- exchange resins in the water industry in recent decades. In this study, high sulfate removal efficiency （80%-90%） was achieved at the influent sulfate concentration of 3600 mg/L and 3% NaC1 after 145 days in an expanded granular sludge bed （EGSB） reactor. Furthermore, the feasibility of treating synthetic waste brine containing high levels of sulfate and nitrate was investigated in a single EGSB reactor during an operation period of 261 days. The highest nitrate and sulfate loading rate reached 6.38 and 5.78 kg/（m3-day） at SO42--S/NO3-N mass ratio of 4/3, and the corresponding removal efficiency was 99.97% and 82.26% at 3% NaC1, respectively. Meanwhile, 454-pyrosequencing technology was used to analyze the bacterial diversity of the sludge on the 240th day for stable operation of phase X. Results showed that a total of 9194 sequences were obtained, which could be affiliated to 14 phyla, including Proteobacteria, Firmicutes, Chlorobi, Bacteroidetes, Synergistetes and so on. Proteobacteria （77.66%） was the dominant microbial population, followed by Firmicutes （12.23%） and Chlorobi （2.71%）.The disposal of waste brines has become a major challenge that hinders the wide application of ion- exchange resins in the water industry in recent decades. In this study, high sulfate removal efficiency （80%-90%） was achieved at the influent sulfate concentration of 3600 mg/L and 3% NaC1 after 145 days in an expanded granular sludge bed （EGSB） reactor. Furthermore, the feasibility of treating synthetic waste brine containing high levels of sulfate and nitrate was investigated in a single EGSB reactor during an operation period of 261 days. The highest nitrate and sulfate loading rate reached 6.38 and 5.78 kg/（m3-day） at SO42--S/NO3-N mass ratio of 4/3, and the corresponding removal efficiency was 99.97% and 82.26% at 3% NaC1, respectively. Meanwhile, 454-pyrosequencing technology was used to analyze the bacterial diversity of the sludge on the 240th day for stable operation of phase X. Results showed that a total of 9194 sequences were obtained, which could be affiliated to 14 phyla, including Proteobacteria, Firmicutes, Chlorobi, Bacteroidetes, Synergistetes and so on. Proteobacteria （77.66%） was the dominant microbial population, followed by Firmicutes （12.23%） and Chlorobi （2.71%）.

关 键 词：454-pyrosequencing waste brine denitrification expanded granular sludge bed （EGSB） reactor 

分 类 号：X703[环境科学与工程—环境工程] X783.03