伴性赤蚁基因(sch)的RAPD分子标记研究  

STUDIES ON THE MOLECULAR MARKER OF THE SEX-LINKED CHOCOLATE GENE(sch )IN SILKWORM WITH RAPD

作　　者：高贵田[1] 朱勇[1] 陈萍[1] 代方银[1] 鲁成[1] 

机构地区：[1]西南农业大学蚕桑丝绸学院农业部蚕桑学重点开放实验室,安康农校400716

出　　处：《蚕学通讯》2000年第4期1-3,共3页Newsletter of Sericultural Science

基　　金：国家自然科学基金重点项目(39730730);国家教委博士点专项基金(960602)资助。

摘　　要：用夏芳♂、夏ｓｃｈ♂、秋白♂、秋ｓｃｈ♂两对近等位基因系为材料，采用随机引物进行 ＰＣＲ扩增。通过９０个随机引物，在夏ｓｃｈ♂中得到ＯＰＤ０２－４００ｂｐ多态性特异带，用秋白、秋ｓｃｈ进 一步验证确认：ＯＰＤ０２－４００ｂＰ是ｓｃｈ基因的 ＲＡＰＤ标记。将获得的分子标记 ＤＮＡ片段回收纯化 后，克隆到质粒ｐＢｌｕｅｓｃｒｉｐｔ－ＩＩＳＫ载体上。PCR amplification was made with arbitrary primers, using two pairs of near - isogenic lines(Xiafang ♂ and Xia sch ♂, and Qiubai ♂ and Qiu sch ♂ ) of silkworm as the experiment materials. A polymor- phic specific band, OPD02 - 400bp, was obtained with 90 arbitrary primers in Xia Sch ♂. A further ex- periment with Qiubai and Qiu sch confirmed that OPD02 - 400bp is the PAPD marker for the sex - linked chocolate gene sch. The DNA fragments of the molecular marker were recovered, purified and cloned onto the carrier - plasmid pBluescript - II SK.

关 键 词：家蚕 PAPD 分子标记 伴性赤蚁基因 温敏性 性别控制 雄蚕 

分 类 号：S882[农业科学—特种经济动物饲养]