蒙古黄芪植株水浸液的自毒作用研究  

Autotoxicity of Aqueous Extracts from Plant of Cultivated Astragalus membranaceus var. mongholicus

作　　者：张新慧[1,2] 郎多勇[3] 陈靖[1,2] 赵云生[1,2] 吴秀丽[1,2] 付雪艳[1,2] 

机构地区：[1]宁夏医科大学药学院,宁夏银川750004 [2]宁夏回药现代化工程技术研究中心/宁夏回医药协同创新中心,宁夏银川750004 [3]宁夏医科大学实验动物中心,宁夏银川750004

出　　处：《中药材》2014年第2期187-191,共5页Journal of Chinese Medicinal Materials

基　　金：国家科技支撑计划子课题(2011BAI05B05)

摘　　要：目的:通过对蒙古黄芪植株各部位提取液的生物学试验,探讨蒙古黄芪是否存在自毒作用,以及自毒作用与连作障碍的关系。方法:设置蒸馏水(CK)、蒙古黄芪根、茎和叶水浸提液各12.5、25、50和100 mg/mL 4个浓度,共计13个处理,用于黄芪自毒作用的生物学实验。结果:当蒙古黄芪根、茎和叶水浸提液的浓度达到25 mg/mL时,对自身种子萌发和幼苗生长有抑制作用,且随水浸提液浓度的升高,这种抑制作用增强;就综合化感效应而言,茎水浸提液的抑制作用>叶水浸提液>根水浸提液。发芽指数和幼苗活力指数对水浸提液比其他指标更为敏感。结论:蒙古黄芪植株水浸提液对自身种子萌发和幼苗生长具有明显的抑制作用,且抑制作用具有一定的浓度依赖性,蒙古黄芪自毒作用是造成连作障碍的原因之一。Objective：To exploring the relationship between continuous cropping obstacle and autotoxicity of Astragalus membrana- ceus var. mongholicus, autotoxic effect of plant aqueous extract were determined. Methods ： Distilled water （ CK ）, aqueous extract of plant,including root, stem and leaf＇（ 12.5,25,50 and 100 mg/mL respectively）were applied to testing their effect on early growth of As- tragalus membranaceus var. mongholicus. Specifically, seed germination rate, germination index, emergence rate, ehmgation of radical and embryo,and seedling vigor index were determined. Results ：The aqueous extract of root, stem,and leaf at 25 mg/mI, significantly inhibi- ted the seed germination and seedling growth of Astragalus membranaceus vat. mongholicus, and this inhibitory effect generally increased with the increase of the concentration of aqueous extracts. To the comprehensive allelopathic effect,the extracts from Astragalus membrn- naceus var. mongholicus stem were more inhibitory than those from leaf and root. The germination index and seedling vigor index were more sensitive to extract than other determined parameters. Conclusion： Aqueous extracts from Astragalus membramweus var. mongholicus plant gave inhibitory effects on Astragalus. membrnnaceus vat. mongholicus germination and seedling growth, and this inhibitory effect generally increased with the increases of aqueous extract concentration at a certain ranges. In conclusion,there is an autotoxicity in con- tinuous cropping of Astragalus membranaceus vat. mongholicus.

关 键 词：蒙古黄芪 自毒作用 植株 水浸提液 种子发芽 幼苗生长 

分 类 号：R282.2[医药卫生—中药学]