穿心莲二萜内酯类成分的高效薄层色谱指纹图谱研究  

HPTLC Fingerprint Analysis of Andrographolides from Andrographis paniculata

作　　者：邵艳华[1] 王建刚[1] 赖小平[1] 吴向维[1] 丁平[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中药材》2014年第2期219-223,共5页Journal of Chinese Medicinal Materials

基　　金："十二五"国家科技支撑计划子课题(2011BAI01B01);广东省科技计划项目(2012A030100010)

摘　　要：目的:建立穿心莲二萜内酯类成分的高效薄层荧光色谱指纹图谱,并对不同种植基地穿心莲、不同部位及市售饮片等进行分析比较。方法:应用硅胶GF254高效预制薄层板(20 cm×10 cm),以氯仿-甲苯-甲醇(80∶10∶15)为展开剂,于相对湿度42%左右展开,5%硫酸乙醇溶液喷雾显色,105℃加热至斑点显色清晰,置紫外光灯(366 nm)下检视。将所得薄层荧光色谱指纹图谱经指纹图谱系统解决方案软件(CHROMA 1.5)生成共有模式。结果:穿心莲二萜内酯类成分指纹图谱由9个色谱峰(或特征荧光条斑)组成,并指认了其中的穿心莲内酯、脱水穿心莲内酯及新穿心莲内酯3个峰。51批不同穿心莲药材样品的HPTLC考察结果显示,不同种植基地、不同商品穿心莲药材以及穿心莲不同部位等在二萜内酯类成分方面区别较大;干燥处理对穿心莲内酯和穿心莲总内酯含量变化的影响差别显著。结论:该方法简便、快速,可有效地对穿心莲药材进行鉴别及质量评价。Objective：To establish the high-performance thin layer chromatography （HPTLC）fingerprint of andrographolides from Andrographis paniculata, and to valuate the fingerprint similarity of samples from different habitats, markets, used parts and so on. Meth- ods ： Chromatographic conditions were as follows ： stationary phase ： precoated HPTLC GF254 silica-gel plate （20 cm × 10 cm） ; developing solvent system ： chloroform-toluene-methanol （ 80：10：15 ） ; Relative humidity ：42 % ; Color development reagent ：5 % H2 SQ ethanolic so- lution ,heating at 105 ℃ and observing the fluorescent chromatogram in a UV cabinet at 366 nm. The common patterns of HPTLC finger- print were obtained through CHROMAP 1.5 solution software. Results：The HPTLC fingerprint of andrographolides was consisted of 9 characteristic peaks（ fluorescent bands）including andrographolide, neoandrographolide and dehydroandrographolide which were chemical reference substances. The investigation and analysis of 51 batches of Andrographis paniculata showed that there were remarkable differ- ences among different samples, so was the content of andrographolide and total lactones. Conclusion ： This method is simple and rapid, which can serve as an effective identification and quality assessment method for Andrographis paniculata.

关 键 词：穿心莲 二萜内酯 高效薄层荧光色谱指纹图谱 

分 类 号：R282.5[医药卫生—中药学]