枇杷花药材HPLC指纹图谱研究  

Study on HPLC Fingerprint of Eriobotrya japonica Flower

作　　者：李琪[1,2] 李慧敏[1] 杨必坤[1] 张宏[1,2] 刘刚[1,2] 

机构地区：[1]四川师范大学生命科学学院,四川成都610068 [2]四川师范大学植物资源应用与开发研究所,四川成都610068

出　　处：《中药材》2014年第2期224-227,共4页Journal of Chinese Medicinal Materials

基　　金：四川省科技厅第三批应用技术研究与开发项目(2009SZ005);四川省教育厅青年基金项目(2006B035)

摘　　要：目的:建立枇杷花药材HPLC指纹图谱分析方法。方法:采用Alltech Apollo C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.1%冰醋酸溶液梯度洗脱,流速:0.5 mL/min,检测波长:210 nm,柱温:20℃,以熊果酸为参照物,对13批不同产地枇杷花药材进行指纹图谱分析。结果:建立了枇杷花药材的HPLC指纹图谱,标定出共有峰17个,并用对照品指认了2个峰,13批不同产地枇杷花相似度存在差异。结论:根据该色谱条件构建的HPLC指纹图谱为枇杷花药材质量评价提供了科学依据。Objective ： To establish an HPLC fingerprint of Eriobotrya japonica flower. Methods ： 13 batches of Eriobotrya japonica flowers from different habitats were analyzed by HPLC with ursolic acid as reference substance. The chromatographic condition was per- formed on klltech Apollo C,s column（250 trim × 4. 6 mm ,5 μm） , eluted with mobile phase containing methanol and O. 1% glacial ace- tic acid in gradient mode. The flow rate was 0. 5 mL/min and the detection wavelength was set at 210 nm. The temperature of column was 20 ℃. Result：The HPLC fingerprint of Eriobotrya japonica flower had been established. There were 17 common peaks, two of which were identified by reference substances. 13 batches of sample from different habitats can be distinguished from their fingerprints. Conclusion：This method is reasonable and reliable, which can be used for quality evaluation of Eriobotrya japonica flower.

关 键 词：枇杷花 HPLC指纹图谱 相似度分析 

分 类 号：R282.5[医药卫生—中药学]