化痰降气方对人支气管上皮细胞多药耐药相关蛋白1的影响  

Influence of Huatanjiangqi Prescription on Multidrug Resistance-associated Protein 1 in Human Bronchial Epithelial Cells

作　　者：汪辰吟[1] 汪电雷[1] 陶秀华[1] 杨丽丽[1] 陈金佩[1] 曹银[1] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230038

出　　处：《中药材》2014年第2期280-283,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(81001592);教育部科学技术重点项目(210101);安徽高校省级自然科学研究重点项目资助(KJ2010A210;KJ2012A186);国家中医药管理局重点学科中医肺部学学科开放基金项目(2011fbxk015B)

摘　　要：目的:研究化痰降气方对人支气管上皮细胞上多药耐药相关蛋白1功能和表达的影响。方法:体外培养人支气管上皮细胞16HBE14o-,以5-羧基二乙酸荧光素(5-CFDA)为底物,采用流式细胞术检测给予化痰降气方后细胞内荧光强度的变化评价MRP1功能;real time RT-PCR测定给予化痰降气方后MRP1 mRNA的变化。结果:化痰降气方在一定浓度范围内能促进16HBE14o-增殖;与5-CFDA模型组比较,低、中、高浓度(100、1 000、2 000μg/mL)的化痰降气方对MRP1功能分别提高22.59%、47.14%、68.36%;化痰降气方能浓度依赖性诱导16HBE14o-细胞MRP1 mRNA表达的量,中、高浓度诱导作用有显著性差异(P<0.05)。结论:化痰降气方能提高16HBE14o-MRP1外排功能和mRNA的含量,且呈一定的浓度依赖性。Objective：To investigate the effect of Huatanjiangqi prescription（Sinapis Semen,Perillae Fructus, Cynanchi Stauntonii Rhizoma et Radix ,lnulae Herha, Angelieae Sinensis Radix, Honey-fi＇ied Ephedrae Herha） on multidrug resistance-associated protein l （MRPI） in human bronchial epithelial cells. Methods ：The human bronchial epithelial cells line 16HBE14o- was used to analyze the in vitro effect of Huatanjiangqi prescription on MRP1 transport. 5-CFDA was used as a model MRP1 substrate and was measured with flow cytometry. The mRNA expression of MRP1 was detected by real-time PCR. Resuhs：Huatanjiangqi prescription could promote the prolif- eration of human bronchial epithelial cells 16HBE14o- in a certain range of concentration;Compared with the control group（5-CFDA） , low,medium and high concentration （ 100,1 000,2 000 μg/mL） of Huatanjiangqi prescription on MRPI function were increased by 22.59% ,47. 14% and 68.36%, respectively; Huatanjiangqi prescription could concentration-dependently induce the expression of MRP1 mRNA,medium and high concentration could induce a significant difference. Conclusion：Huatanjiangqi prescription can improve MRP1 efflux function and mRNA levels in a concetration-dependent manner.

关 键 词：多药耐药相关蛋白1 化痰降气方 慢性阻塞性肺疾病 人支气管上皮细胞 

分 类 号：R285.5[医药卫生—中药学]