辣椒制品中罗丹明B的基质固相分散提取条件优化  

Matrix Solid-Phase Dispersion Extraction Conditions of Rhodamine B from Chili Products

作　　者：韦娜[1,2] 王煜 张孝芳[1] 冯叙桥[2] 齐小花[1] 邹明强[1] 

机构地区：[1]中国检验检疫科学研究院装备技术研究所,北京100123 [2]沈阳农业大学食品学院,辽宁沈阳110161 [3]国家认可中心,北京100062

出　　处：《食品与生物技术学报》2014年第3期275-281,共7页Journal of Food Science and Biotechnology

基　　金：国家"十二五"科技支撑计划项目(2012BAK25B01;2012BAK08B06-1)

摘　　要：采用改进的基质固相分散提取技术提取辣椒制品中的罗丹明B,对提取工艺进行优化。选择分散剂种类、分散剂与样品质量比、洗脱剂组成及洗脱剂用量作为研究因素,以提取液中罗丹明B的含量为考察指标,运用L16(45)正交试验优选出了辣椒面中提取罗丹明B的最优工艺条件:分散剂硅胶,分散剂与样品质量比例1∶1;洗脱剂为质量分数80%乙腈水溶液,洗脱剂用量20 mL。辣椒油中提取罗丹明B最优工艺条件是:分散剂中性氧化铝,分散剂与样品质量比例2∶1;洗脱剂为体积分数80%甲醇水溶液,洗脱剂用量20 mL。辣椒酱中提取罗丹明B最优工艺条件是:分散剂中性氧化铝,分散剂与样品质量比例3∶1;洗脱剂为质量分数80%乙腈水溶液,洗脱剂用量5 mL。该提取方法萃取工艺简单,操作时间短,所需有机溶剂种类少,属环境友好型萃取方式。with the Rhodamine B content in extract as index,the optimum parameters,including dispersant type,the ratio of dispersing agent and sample,composition of the eluent and elute volume,of extracting Rhodamine B from chili were investigated by using orthogonal design L16（45）.The optimum conditions for extracting Rhodamine B from chili powder are as follows：dispersant type is silica gel,the ratio of dispersing agent and sample is 1∶1,composition of the eluent is 80% of acetonitrile,elute volume is 20 mL.The optimum conditions for extracting Rhodamine B from pepper oil are as follows：dispersant type is neutral aluminum oxide,the ratio of dispersing agent and sample is 2∶1,composition of the eluent is 80% of methanol,elute volume is 20 mL.The optimum conditions for extracting Rhodamine B from chili paste are as follows：dispersant type is neutral aluminum oxide,the ratio of dispersing agent and sample is 3∶1,composition of the eluent is 80% of acetonitrile,elute volume is 5 mL.The extraction method is simple,convenient,less material consumption and less organic solvent.

关 键 词：罗丹明B 正交试验 基质固相分散萃取 

分 类 号：TS255.5[轻工技术与工程—农产品加工及贮藏工程]