温阳消饮法对胸腔积液大鼠小肠水通道蛋白4表达的影响  

Effect of yang-warming and fluid retention-resolving therapy on expression of aquaporin-4 in small intestine among rats with pleural effusion

作　　者：赵欣悦[1] 张琦[1] 赖宇[1] 李思思[1] 肖桦[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《湖南中医杂志》2014年第3期117-118,共2页Hunan Journal of Traditional Chinese Medicine

基　　金：教育部高等学校博士学科点专项科研基金(编号:20115132110008)

摘　　要：目的:研究温阳消饮法对胸腔积液模型大鼠小肠水通道蛋白4(AQP4)表达的影响。方法:采用计算机随机分组方法,将SD成年雄性大鼠分为温阳消饮组、模型对照组、空白对照组,每组12只。对除空白对照组外的其余2组动物以1%角叉菜胶胸腔注射建立大鼠胸腔积液(悬饮)模型,造模24h后拍X线片对模型进行病理评价。于造模成功后2h各组予相对应药物灌胃,每天1次,连续4d。灌胃结束后处死各组大鼠,采集小肠组织,用免疫组化法检测AQP4的表达,比较各组结果。结果:与空白对照组比较,模型对照组表达降低(P<0.05);温阳消饮组高于模型对照组(P<0.05),与空白对照组比较差异无统计学意义(P>0.05)。结论:温阳消饮法可上调胸腔积液模型大鼠小肠AQP4的表达。Objective ： To study the effe,t of yang - warming and fluid retention - resolving therapy （YWFRRT） on the expression of aquaporin -4 （AQP4） in the small intestine among rats with pleural effusion. Methods：Aduh male Sprague -Dawley rats were digital- ly randomized into YWFRRT group （ n = 12 ）, model control group （ n = 12 ）, and blank control group （ n = 12 ）. All rats except those in blank eontrol group received intrapleural injection of 1% carrageenan to establish a rat model of pleural effusion （ pleural fluid reten- tion）. At ：24 h after the model was established, model rats were subjected to pathological evaluation by radiography. At 2 h after the model was established, each group received respective treatments by garage once daily for 4 d. All rats were sacrificed after intragastric administration,and small intestine tissues were collected. The expression of AQP4 was measured by immunohistochemistry,and the re- suits were compared between groups. Resuhs：Compared with the blank control group,the model control group showed significant de- crease in AQP4 expression（P 〈 0. 05 ） ;the YWFRRT group had significantly higher AQP4 expression than the model control group （ P 〈 0. 05 ） , but there was no significant difference between the YWFRRT group and blank control group （ P 〉 0. 05 ）. Conclusion ： YWFRRT can up - regulate AQP4 expression in the small intestine among mice with pleural eflusion.

关 键 词：胸腔积液 大鼠 温阳消饮法 水通道蛋白4 实验研究 

分 类 号：R285.5[医药卫生—中药学]