益肾通络方对大鼠的急性毒性研究  

Study on acute toxicity of kidney-nourishing and collateral-dredging prescription in rats

作　　者：邹晨曦[1] 金一[1] 杨智[1] 凌晓[1] 金红[1] 卢岳华[1] 

机构地区：[1]湖南师范大学医学院,湖南长沙410006

出　　处：《湖南中医杂志》2014年第3期122-123,共2页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助项目(编号:14JJ4033);湖南省中医药科研计划项目(编号:2013106);湖南省教育厅资助项目(编号:12A098);湖南省大学生研究性学习和创新性实验计划项目(编号:201310542011)

摘　　要：目的:观察益肾通络方对大鼠的急性毒理作用。方法:将40只SD大鼠随机分为受试组和对照组,每组20只,受试组大鼠以益肾通络方汤剂最大浓度灌胃给药,空白对照组灌服相同体积的蒸馏水,连续14d;观察大鼠死亡数及益肾通络方对大鼠的体质量和脏器的影响。结果:大鼠无死亡现象,益肾通络方对肝、脾、肾、胃、肠、心、肺等主要脏器无明显影响;未测出LD50;其最大给药量为150g/kg体质量,相当于临床剂量的64倍,未出现异常的毒性反应。结论:益肾通络方具有较高的安全性。Objective ：To observe the acute toxicity of kidney -nourishing and collateral -dredging prescription in rats. Methods： Forty Sprague - Dawley rats were randomly divided into test group （ n = 20） and control group （ n = 20）. The test group received the highest concentration of kidney - nourishing and collateral - dredging prescription （decoction） by garage, while the control group re- ceivedan equal volume of distilled water;the course of treatment was 14 d. The mortality of＂ rats and the effects of kidney -nourishing andcollateral - dredging prescription on the body weight and visceral organs of rats were determined. Results ： No mortality was found in the rats. Kidney - nourishing and collateral - dredging prescription showed no significant effects on major organs such as the liver, spleen, kidney, stomach, intestine ,heart, and lung. The median lethal dose of kidney- nourishing and collateral -dredging prescrip- tion was not determined. The maximum dose of kidney - nourishing and collateral - dredging prescription was 150 g/kg body weight, 64 times the clinical dose, and toxic reactions were not observed. Conclusion ： Kidney - nourishing and collateral - dredging prescrip- tion has high safety.

关 键 词：益肾通络方 急性毒性 大鼠 实验研究 

分 类 号：R285.1[医药卫生—中药学]