缺血后适应经PI3K-AkteNOS保护成年老年大鼠心肌缺血-再灌注损伤的研究  

Ischemic postconditioning protects both adult and aged SD rat hearts from ischemia-reperfusion injury through the PI3K/Akt and eNOS pathway

作　　者：曹阳[1] 朱贵家[1] 孙兑荣 陈琳[1] 杨树森[1] 

机构地区：[1]哈尔滨医科大学附属第一医院心内科, 黑龙江 哈尔滨150001

出　　处：《中国急救医学》2014年第4期361-366,共6页Chinese Journal of Critical Care Medicine

基　　金：黑龙江省自然科学基金资助项目（D201105）

摘　　要：目的：探讨信号通路Akt及eNOS与缺血后适应对成年、老年大鼠急性缺血-再灌注损伤心肌保护作用的关系。方法利用Langendorff灌注系统建立体外大鼠心肌缺血-再灌注损伤模型，应用多导电生理仪记录各组缺血及再灌注过程中的CF、HR、LVDP、＆#177;dp/dtmax值，应用伊文思蓝（ Evans blue ）和TTC双染色测定心肌梗死面积。应用Western blot法检测心肌内Akt、P-Akt、eNOS、P-eNOS的表达水平。结果①成年、老年缺血后适应组（ IPostC）较缺血组（ IRI）心脏功能（LVDP和＆#177;dp/dtmax）明显改善（P＜0．05）。②成年、老年后适应组（IPostC）较缺血组（IRI）心肌梗死面积明显缩小（P＜0．05）。③成年、老年后适应组（IPostC）中P-Akt和P-eNOS表达水平较缺血组（IRI）明显上调（P＜0．05）。结论①缺血后适应对成年和老年缺血-再灌注损伤均有保护作用。②再灌注早期给予后适应操作可激活PI3 K/Akt/eNOS信号通路而减轻急性心肌缺血-再灌注损伤。Objective To study the protective effect of ischemic post -conditioning on rat＆#39;s acute myocardial ischemia/reperfusion （ I/R） injury and the mechanism of cardiac protection through Akt and eNOS.Methods Using the Langendorff reperfusion system to establish an ex vivo rat model of whole heart I/R.Multi-conductive physiological recorder was used to record cardiac function as CF , HR, LVDP, ＆#177;dp/dtmax of all groups were recorded .Six rat hearts of each group were used to determine myocardial infarct size by dual staining with triphenyltetrazolium chloride and Evan ＆#39;s blue dye. The other rats of each group were saved for Western blotting analysis as the expression of Akt , P-Akt, eNOS and P-eNOS.Results ①The cardiac function （ LVDP and ＆#177;dp/dtmax） of adult/aged IPostC group was significantly better than that of the IRI group （P＆lt;0.05）.②The infarction area of adult/aged IPostC group was smaller than that of the IRI group （P＆lt;0.05）.③The expression of P -Akt and P-eNOS of all adult/aged IPostC groups was significantly higher than that of the IRI groups （P＆lt;0.05）. Conclusion ①Ischemic post -conditioning protects the adults or aged rats from the I /R injuries.②Given the postcondition dispose at the very beginning of the reperfusion can alleviate the acute cardiac I/R injuries through activation of PI 3K-Akt and eNOS kinase .

关 键 词：心肌缺血-再灌注 老年 后适应 一氧化氮 

分 类 号：R542.2[医药卫生—心血管疾病]