紫花苜蓿EMS突变体库的构建和形态学性状鉴定  

Construction of an EMS induced mutant library and identification of morphological characteristics in Medicago sativa

作　　者：夏曾润[1] 杜凤凤[1] 李偲[1] 张吉宇[1] 刘勇[1] 霍雅馨 孔令芳[1] 

机构地区：[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2014年第2期215-222,共8页Acta Prataculturae Sinica

基　　金：国家自然科学基金(31101759);甘肃省农业生物技术研究与应用开发项目(GNSW-2011-16);兰州大学中央高校基本科研费自由探索项目(lzujbky-2013-87)资助

摘　　要：通过不同浓度的EMS(甲基磺酸乙酯)溶液诱变处理紫花苜蓿种子,系统分析了EMS对M1代种子萌发和生长的影响,对M1代突变植株的形态学性状进行了初步鉴定。结果表明,EMS处理抑制了M1代种子的最终发芽率、发芽指数和胚根胚芽长度。初步鉴定了M1代1039个单株的形态学性状,共获得378份叶(不含叶形和叶数)、株高、分枝数、株型以及其他性状发生变异的突变株,其中叶色、分枝数、株型和其他性状突变体依次为215,40,95和28株,突变率依次为20.69%,3.85%,9.14%,2.69%。本研究初步构建的紫花苜蓿EMS突变群体为苜蓿功能基因组研究、遗传改良及育种奠定了基础。The effects of different concentrations of EMS on seed germination and seedling growth in Medicago sativa were analysed and the morphological characters of M1 mutants identified. The final germination rate, germination index and the length of radicle and germ of M1 seeds were restrained after EMS treatment. There were 379 mutants from 1039 M1 populations showing leaf （but excluding leaf shape and quantity）, plant height, branch number, plant type mutations. The mutant lines of leaf color, branch number, plant type and others were 215, 40, 95 and 28 respectively and the frequency was 20. 690/00, 3.85G, 9.14G and 2.69G. The mutant library we constructed will be a very useful genetic resource for functional genomics and genetic improvement of M. sativa.

关 键 词：紫花苜蓿 EMS(甲基磺酸乙酯) 半致死剂量 突变体库 变异性状 

分 类 号：S541.1[农业科学—作物学]