甲基磺酸乙酯诱变选育竹红菌甲素高产菌株  

Breeding of Shiraia sp. strain with high-yield hypocrellin by EMS mutation

作　　者：杨珠英[1] 潘魏松[1] 聂云[1] 罗晗[1] 王剑文[1] 

机构地区：[1]苏州大学医学部药学院,苏州215123

出　　处：《生物加工过程》2014年第2期24-27,共4页Chinese Journal of Bioprocess Engineering

基　　金：教育部留学基金(K513201011);国家大学生创新创业训练计划(5731502710);大学生课外学术科研基金(KY2013558B)

摘　　要：以从短穗竹（ Brachystachyum densiflorum）茎秆中分离获得的竹黄菌（ Shiraia sp.S8）菌株为出发菌株，以.5％～2.5％甲基磺酸乙酯（ EMS）处理竹黄菌悬浮孢子～30 min，结合平板初筛和高效液相色谱（ HPLC）分析进行复筛。经诱变筛选得到高产菌株，发现其竹红菌甲素产量达到.8 mg/L，与原始出发菌株相比提高了.4％，且遗传稳定性良好，具有较高的医药及工业应用价值。One isolated strain Shiraia sp. S8 from the stems of Brachystachyum densiflorum was breeding candidates for higher production of hypocrellin A （ HA） by ethyl methane sulphonate （ EMS） mutation. EMS at 0.5% to 2.5% was used to treat the suspension spores for 10 min to 30 min.Through the combined methods with the preliminary screening on the diameter of the red pigment ring and HPLC,the mutant 10-5 with higher HA yield was obtained and the production was verified to be genetically stable.HA yield by mutant 10-5 was increased by 46.4% than that of the original strain.The EMS mutant had industrial potential and pharmaceutical importance for HA.

关 键 词：甲基磺酸乙酯 竹红菌素 选育 竹黄菌 

分 类 号：Q784[生物学—分子生物学]