罗丹明汞离子荧光探针的合成及光谱研究  

Synthesis and Spectrum of Mercury Ion Fluorescent Probe Based on Rhodamine Dyes

作　　者：张丽珠[1] 于璐[1] 梁坤[2] 谷利军[1] 樊保敏[1] 杨丽娟[1] 

机构地区：[1]云南民族大学国家民委-教育部民族药资源重点实验室,云南昆明650500 [2]西南林业大学理学院,云南昆明650224

出　　处：《石油化工》2014年第4期461-465,共5页Petrochemical Technology

基　　金：国家自然科学基金项目(21162040;21162042)

摘　　要：通过硫的亲汞性和氨基硫脲衍生物能被Hg2+转化为胍衍生物的机理,合成了一种新的Hg2+探针3′,6′-二乙氨基-2,7-二甲基-2-(N-氨基乙基)螺[异吲哚-1,9′-呫吨]-3-硫酮(R6G1)。考察了探针R6G1的水溶性、光谱性能及其在水相中对常见离子的选择性,测定了探针R6G1与Hg2+的络合比例和络合常数。水溶液中金属阳离子选择性测试结果表明,探针R6G1的水溶性好,能高选择性地识别Hg2+,该识别过程不受Fe3+,Cu2+,Al3+,Cr3+,Fe2+,Mn2+,Co2+,Pb2+,Mg2+,Ca2+,Cd2+,Zn2+,Ba2+等金属阳离子和F-,Cl-,Br-等阴离子的干扰。紫外滴定结果表明,探针R6G1与Hg2+按1∶1的化学计量比络合。荧光滴定测试结果表明,探针R6G1识别Hg2+的络合常数lgKa=5.92。A Hg2＋ fluorescence probe,3＇,6＇-diethylamino-2,7-dimethyl-2-（N-aminoethyl）spiro [isoindoline-1,9＇-xanthene]-3-thione（R6G 1）,was synthesized through the strong thionic affinity with Hg2＋ and the transformation of thiourea derivatives to guanidine derivatives by the promotion of Hg2＋.The water solubility,spectrum and ion selectivity of R6G1 were investigated.The complexing ratio of Hg2＋ to R6G1 and the complexation constant were measured through fluorometric titration.The results showed that the probe R6G1 had high selectivity to Hg2＋ and good water solubility.The recognition process of R6G1 to Hg2＋ was not affected by other metal ions such as Fe3＋,Cu2＋,Al3＋,Cr3＋,Fe2＋,Mn2＋,Co2＋,Pb2＋,Mg2＋,Ca2＋,Cd2＋,Zn2＋,Ba2＋,and anions such as F,Cl-,Br.Spectrophotometric titration indicated that the complex of Hg2＋ and R6G1 formed with the ratio of 1 ∶ 1 and the complexation constant（lgKa） was 5.92.

关 键 词：罗丹明 荧光探针 汞离子 光谱性能 

分 类 号：TQ426.91[化学工程]