浓缩自体骨髓移植结合补肾活血中药对骨质疏松性骨折愈合的影响  

Effect of Concentrated Autologous Bone Marrow Aspirate Transplantation Combined With KidneySupplementing Blood-Quickening Herbs on Fracture Healing in Osteoporotic Fractures

作　　者：王昌兴[1] 董黎强[1] 刘琦[1] 何键能 姜滔[1] 赵友[1] 

机构地区：[1]浙江中医药大学附属第二医院

出　　处：《中医杂志》2014年第8期699-702,共4页Journal of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y2080991)

摘　　要：目的探讨自体骨髓移植结合补肾活血中药治疗骨质疏松性骨折(OPF)的作用机制。方法 100只大耳白兔随机分为假手术组、模型组、移植组、中药组和联合组各20只。除假手术组外其余各组采用双侧卵巢切除3个月方法建立骨质疏松症模型,各组再行左前肢锯断桡骨3mm建立骨折模型。移植组将自体浓缩骨髓(2ml/只)经皮注射到骨折断端;中药组给予补肾活血中药10ml/(kg·d)灌胃;联合组给予骨髓移植和补肾活血中药灌胃,剂量同上;假手术组、模型组给予同量的生理盐水灌胃,共8周。分别于治疗2、4、6、8周对骨折处骨组织进行大体观察、X线摄片及CT平扫,测定骨痂CT值,并进行HE染色观察形态学变化。结果联合组、移植组及中药组从大体观察、X线、HE染色均明显好于模型组,并且联合组均优于其他各组。与假手术组比较,模型组白兔2、4、6、8周时骨折端骨痂CT值明显降低(P<0.01);除移植组8周外,移植组和联合组2、4、6、8周时骨折端骨痂CT值明显高于中药组(P<0.01);联合组4、6、8周时骨折端骨痂CT值高于移植组(P<0.01)。结论浓缩自体骨髓移植结合补肾活血中药能够促进加快OPF的愈合,并且优于单纯应用补肾活血中药或浓缩自体骨髓移植治疗。Objective To study the effect of concentrated autologous bone marrow aspirate transplantation （CABMAT） combined with kidney-supplementing blood-quickening herbs on fracture healing in osteoporotic fractures （OPF）. Methods Totally 100 white rabbits were randomized into the sham operation group, model group, transplantation group, Chinese medicinal group and combination group, with 20 in each. The osteoporotic models were established by ovariectomy for three months in all groups except the sham operation group. Then the fracture models were established by amputating 3mm radial bones of left forelegs in all groups. The concentrated autologous bone marrow （2ml/rabbit） was percutaneously injected into the fracture fragments in the transplantation group. The Chinese medicinal group was garaged with 10ml/ （kg ~ d） kidney-supplementing blood-quickening herbs. The combination group was given bone marrow transplantation and intragastric administration of kidney-supplementing blood-quickening herbs. The sham operation group and model group were gavaged with the same amount of normal saline for 8 weeks. The general observation, X-ray and CT scanning were conducted in the bone tissue of fracture in the 2nd, 4th, 6th and 8th week of treatment. The CT values of bone callus were measured and the morphological changes were observed with HE staining. Results The combination group, transplantation group and Chinese medicinal group were superior to the model group in the general observation, X-ray and HE staining, especially the combination group. Compa- ring with the sham operation group, the CT values of bone callus were significantly decreased in the model group in the 2nd, 4th, 6th and 8th week of treatment （P 〈0.01 ）. Comparing with the Chinese medicinal group, the CT values of bone callus were significantly increased in the transplantation group and combination group in the 2nd, 4th, 6th and 8th week of treatment except in the transplantation group in the 8th week of treatment （ P 〈 0.01 ）. Comparin

关 键 词：骨质疏松性骨折 自体骨髓移植 补肾活血中药 

分 类 号：R683[医药卫生—骨科学]