微血管相关因子在强直性脊柱炎患者股骨头骨一软骨及软骨下骨中的表达  

Expression of microvessel associated factors in patients with ankylosing spondylitis in femoral headcartilage and subchondral bone

作　　者：高万里[1] 吴立生[1] 訾金花[1] 张存华[1] 蔡道章[2] 宋炎成[2] 

机构地区：[1]临沂市人民医院创伤外科二病区,山东276003 [2]南方医科大学第三附属医院

出　　处：《中华实验外科杂志》2014年第4期897-899,F0003,F0004,共5页Chinese Journal of Experimental Surgery

基　　金：山东省优秀中青年科学家科研奖励基金资助项目（BS2011YY040）;山东省医药卫生科技发展计划资助项目（2011QW009）;山东省中医药科技发展计划资助项目（2013ZDZK1l55）

摘　　要：目的观察微血管在强直性脊柱炎（As）患者股骨头骨一软骨及软骨下骨中的表达，探讨微血管与AS发病的关系。方法7例AS股骨头标本作为实验组，11例骨关节炎（OA）及9例类风湿关节炎（RA）股骨头标本作为对照组。以抗体检测各标本中血管内皮生长因子（VEGF）、CD56^＋及CD68^＋表达；LeicaQwin高清晰图像分析系统检测灰度值，进行半定量评价。结果As中有软骨覆盖区股骨头表面与OA（12．97±4．31比4．43±1．32；P〈0．01）及RA（12．97±4．31比7．42±3．35；P〈0．05）比较，VEGF在骨．软骨界面表达升高。在骨．软骨连接处的骨质吸收区，AS股骨头有软骨覆盖区与OA（5．14±1．62比1．51±0．93；P〈0．05）及RA（5．14±1．62比0．87±0．60；P〈0．01）比较，CD68^＋表达升高。AS股骨头无软骨覆盖区与有软骨覆盖区比较，骨质破坏灶显著升高（8．04±1．43比5．14±1．62；P〈0．01）。结论在AS中骨一软骨界面和软骨下骨是主要的炎症部位；微血管在诱导炎症过程中起重要作用。Objective To investigate the relationship between microvessel and ankylosing spondy litis （AS） through the expression of microvessel in femoral head bone-cartilage and subchondral bone. Methods Seven cases of femoral head in the patients with AS were used as experimental group, 11 cases of osteoarthritis （OA） and 9 cases of rheumatic arthritisc （RA） were served as control group. To detect antibodies against vascular endothelial growth factor （VEGF）, CD56 ^＋ and CD68 ^＋ expression in all speci- mens, Leica Qwin high definition image analysis system to detect gray value, semi quantitative assessment. Results Our results show that in the femoral head surface with cartilage of AS microvessel in bone and cartilage interface increased compared with OA （ P 〈 0. 01 ） and RA （ P 〈 0. 05 ）. At the osteochondral junction bone absorption region CD68 ^＋ muhinuclear cells significantly increased compared to OA （P 〈 0. 05 ） and RA （P 〈 0. 01 ）. In AS of the femoral head with no cartilage covering surface, bony destruction increased （P 〈 0. 01 ）. Conclusion Bone-cartilage interface and subchondral bone is the site of inflam mation mainly of AS and microvascular may play an important role in the induction of the inflammatory process,

关 键 词：强直性脊柱炎 免疫组织化学 血管内皮生长因子 

分 类 号：R593.23[医药卫生—内科学]