脂肪源性干细胞无酶分离培养方法的研究  

Study on non-enzymatic separation and culture method of adipose-derived stem cells

作　　者：刘苹[1,2] 史春梦[1,2] 胡玲莉 张波[2] 王正国[2] 

机构地区：[1]第三军医大学军事预防医学院复合伤研究所/创伤,烧伤与复合伤国家重点实验室 [2]第三军医大学大坪医院野战外科研究所四室 [3]湖北省孝感市95829部队后勤部门诊部

出　　处：《重庆医学》2014年第10期1153-1156,1160,共5页Chongqing medicine

基　　金：国家重点基础研究发展计划(973)专项课题资助项目(2011CB964701);重庆市科技攻关(重点)资助项目(cstc2012GGB10003)

摘　　要：目的探索适合临床治疗应用的脂肪源性干细胞(ADSCs)分离培养方法。方法分别采用无酶分离法和胶原酶消化法从脂肪抽吸术抽吸的人脂肪组织中分离培养细胞,对比分析分离的间充质干细胞特性。结果无酶分离法所需时间仅为胶原酶消化法的1/3,分离的细胞在细胞形态学、增殖能力、免疫表型、分化潜能等特性与胶原酶消化法分离培养的细胞一致。结论无酶分离法能够从脂肪抽吸术抽吸的人脂肪组织中分离培养出ADSCs,是一种安全可靠、适合临床应用的ADSCs分离培养方法。Objective To explore a separation and culture method of human adipose-derived stem cells（ADSCs） suitable for the clinical application .Methods The non-enzymatic method and the collagenase digestion method were adopted to isolate and culture the cells from the human adipose tissue in the individuals with liposuction .The characteristics of isolated mesenchymal stem cells were comparatively analyzed .Results The required time in the non-enzymatic method was one third of that in the collagenase di-gestion method and the cellular morphology ,reproductive capacity ,immunophenotype and differentiation potential of the isolated cells were consistent to those isolated by the collagenase digestion method .Conclusion The no-enzymatic method may isolate and culture ADSCs from the adipose tissue in the individual with liposuction ,which is a safety and reliable isolation and culture method of human adipose tissue-derived stem cells suitable for clinical application .

关 键 词：脂肪源性干细胞 分离方法 无酶 胶原酶消化法 细胞 培养的 脂肪组织 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]