藜豆花粉的形态及其活力测定方法比较  

Pollen Morphology and Determination of Pollen Activity Methods Comparison of Stizolobium cochinchinesis

作　　者：张凤银[1] 魏传斌[1] 徐小玉[1] 雷刚[1] 

机构地区：[1]江汉大学生命科学学院,湖北武汉430056

出　　处：《贵州农业科学》2014年第3期31-33,共3页Guizhou Agricultural Sciences

基　　金：武汉市科技攻关项目"藜豆引种与高产栽培关键技术研究"(201120822281)

摘　　要：为藜豆的杂交育种及传粉生物学研究等提供基础资料与科学依据,筛选适宜的花粉活力快速测定方法,以白花黑籽藜豆花粉为材料,用光学显微镜观察花粉形态及萌发花粉管的数目,并用TTC法、IKI法和醋酸洋红法对其花粉活力进行了测定。结果表明:成熟的藜豆花粉近似圆球形,有3个萌发孔,但只有1个萌发孔长出花粉管;TTC法着色效果差,I-IK着色时间长(约20min),而醋酸洋红染色法着色快(约2min),染色清晰;醋酸洋红法适合藜豆花粉活力的快速测定。In order to provide basic data and scientific evidence for the study of hybrid breeding and pollination biology,and also to select the suitable method for rapid measurement of life viability,the study was carried out to observe the pollen morphology and number of germination pollen tube of S.cochinchinesis by optical microscope,to observe the pollen morphology using TTC staining method,I-KI staining method and aceto-carmine staining method and to measure the life viability of the velvet bean pollen.The results indicated that the mature pollen was of approximately round ball form and had three germination apertures,but only one of which would grow up with pollen tube.The I-KI staining method and the TTC staining were the inappropriate methods for bad staining method and long staining time（about 20 min）respectively.The method of aceto-carmine staining was the best way to determine the pollen viability of S.cochinchinesis for staining rapidly and ideal staining effect.

关 键 词：藜豆 花粉形态 花粉管 花粉活力 

分 类 号：S567.219[农业科学—中草药栽培]