半夏内生细菌的分离与鉴定  

Isolation and Identification of Endophytic Bacteria from Pinellia ternata

作　　者：张洁[1] 万芬[1] 李玉权[1] 胡琨敏 石建龙[1] 刘红美[1] 

机构地区：[1]贵阳医学院医学生物工程系医学生物技术教研室,贵州贵阳550004

出　　处：《贵州农业科学》2014年第3期94-97,101,共5页Guizhou Agricultural Sciences

基　　金：贵州省中药现代化科技产业研究开发专项项目"贵州半夏块茎腐烂病防治体系关键技术研究与应用示范"[(2013)5014]

摘　　要：为半夏建立有效生物防治和高产优质的绿色种植,采用研磨法和组织块培养法从贵州健康半夏植株的叶、叶柄、块茎中分离纯化到8株内生细菌,并采用细菌16SrDNA序列分析的分子生物学方法结合传统形态和生理生化鉴定方法进行种属鉴定。结果表明:8株内生细菌被鉴定为5属6个种,即芽孢杆菌属的嗜碱芽孢杆菌(Bacillus alcalophilus)、微杆菌属的产左聚糖微杆菌(Microbacterium laevaniformans)、短芽孢杆菌属的土壤短芽孢杆菌(Brevibacillus agri)和热红短芽孢杆菌(Brevibacillus thermoruber)、寡养单胞菌属的嗜麦芽寡养单胞菌(Stenotrophomonas maltrophilia)、类芽孢杆菌属的多黏类芽孢杆菌(Paenibacillus polymyxa)。说明,贵州半夏体内含有多种内生细菌,多数为杆菌,其中芽孢杆菌、多黏类芽孢杆菌等将为半夏益生菌的开发奠定基础。To build a green planting pattern of P .ternata with effective biological control and high yield and quality, eight endophytic strains were isolated and purified by grinding and tissure block culturing methods from tubers,leaves and stipe of health P .ternata in Guizhou.After purification and culturing,endophytic bacteria were identified by 16S rDNA gene （for bacteria）based molecular biology method association with morphological observation and physiological biochemical reactions.The results showed that eight endophytic bacterias were classified into five genera of six families,Bacillus alcalophilus , Microbacterium laevaniformans , Brevibacillus agri , Brevibacillus thermoruber , Stenotrophomonas maltrophilia and Paenibacillus polymyxa . This indicated that various endophytic species existed in P .ternate,and most of them were bacilus.Bacillus sp and Paenibacillus polymyxa isolated from P .ternata would contribute to preparing the probiotic preparations for P .ternata.

关 键 词：半夏 内生细菌 分离鉴定 芽孢杆菌 多黏类芽孢杆菌 

分 类 号：Q93-331[生物学—微生物学]