贵州地方猪骨骼肌中3种microRNAs的表达差异  

Expression Difference of Three microRNAs in the Longissimus Dorsi Muscle of Guizhou Indigenous Pig Breeds

作　　者：刘畅[1,2] 罗志宇[2] 冉雪琴[2] 王嘉福[1] 

机构地区：[1]贵州大学农业生物工程重点实验室,贵州贵阳550025 [2]贵州大学动物科学学院,贵州贵阳550025

出　　处：《贵州农业科学》2014年第3期112-116,共5页Guizhou Agricultural Sciences

基　　金：国家"863"课题"中国地方猪种优异特色性状的功能基因组学研究"(2013AA102503);国家科技支撑计划"高寒山区高效循环养殖(优质猪)关键技术研究与集成示范"(2008BADB5B02);贵州省农业攻关项目"猪繁殖性状候选基因的分离克隆与功能鉴定"[黔科合NY(2013)3073];贵州大学研究生创新基金"地方猪种骨骼肌生长发育miR-133表达调控机制研究"(校研农2013017)

摘　　要：为探明miR-1、miR-133、miR-206基因在不同品种猪肌肉组织中的表达及其与屠宰性能间的关系,以6月龄大白猪、糯谷猪、关岭猪及黔南黑猪的背最长肌为材料,采用实时荧光定量PCR方法,研究了3种microRNAs(miRNAs)在贵州地方猪种和大白猪背最长肌中的表达差异。结果表明:成年期miR-133的表达量高于另外2种miRNA分子。miR-133在糯谷猪中的表达量是大白猪的1.58倍(P<0.05),与其眼肌面积、花油重高度负相关,与屠宰率高度正相关。miR-206在糯谷猪中的表达量是大白猪的2.23倍(P<0.05),与其瘦肉率高度负相关,与板油重中度正相关。在关岭猪背最长肌中miR-133的表达量最高,是大白猪的2.67倍(P<0.05),miR-133和miR-206的表达量与眼肌面积、胴体重高度正相关,与花油重、板油重和脂肪比负相关;但miR-206的表达量明显低于其他猪种。不同品种猪中miR-133和miR-206存在差异表达,对地方猪品种的骨骼肌生长和脂肪沉积有一定的调节作用。To envelope the relationship of miRNAs and muscle growth in pig,the longissimus dorsi muscle were sampled from three indigenous pig breeds,including Nuogu pig,Guanling pig,Qiannan Black pig in 6 months old,taking Yorkshire pig as the control.The expression levels of miR-1,miR-133,miR-206 were analyzed by real-time quantitative PCR detecting system（qPCR）with stem-loop primer for reverse transcription of miRNAs.The results showed that the expression level of miR-133 was higher than both of miR-1 and miR-206 in the longissimus dorsi muscle of pigs.Further,miR-133 level in Nuogu pig was 1.58 times higher than that in Yorkshire pig （P＆lt;0.05）.miR-133 level was negatively correlated with the eye muscle area and abdominal fat weight,while positively correlated with the slaughter of Nuogu pig breed. miR-206 level in Nuogu pig was 2.23 times higher than that in Yorkshire pig （P ＆lt;0.05）,and negatively correlated with the leanness,positively correlated with the leaf fat weight of Nuogu pig.However,the trends of miRNAs change in Guanling pig breed were much different from the others.The expression level of miR-133 was the highest but miR-206 level was the lowest in Guanling pig with 2.67 times higher in miR-133.The levels in both of miR-133 and miR-206 were positively correlated with the eye muscle area and carcass weight of Guanling pig,and negatively correlated with abdominal fat weight,leaf fat weight and fat ratio.miR-133 and miR-206 in different pig breeds had differential expression,which regulated the body growth and fat deposition in indigenous pig breeds of Guizhou Province in certain degree.

关 键 词：MIR-1 miR-133 miR-206 贵州地方猪种 MIR-1 miR-133 miR-206 

分 类 号：S813.3[农业科学—畜牧学]