植物病原真菌寄生性与分泌蛋白组CAZymes的比较分析  

The comparative analysis of secreted CAZymes in phytopathogenic fungi with different lifestyle

作　　者：陈相永[1] 陈捷胤[1] 肖红利[1] 桂月靖[1] 李蕾[1] 戴小枫[1] 

机构地区：[1]中国农业科学院农产品加工研究所,北京100193

出　　处：《植物病理学报》2014年第2期163-172,共10页Acta Phytopathologica Sinica

基　　金：国家重点基础研究发展计划(2013CB127803);国家自然科学基金(31171799;31200113)

摘　　要：通过信息学方法对植物病原真菌分泌蛋白组进行预测以及对碳水化合物酶类CAZymes注释和比较,分析病原真菌寄生性与CAZymes家族功能的关系。结果显示非专性寄生(半活体营养型和死体营养型)真菌编码的分泌蛋白占基因组编码基因的比例较专性寄生真菌(活体营养型)的偏高;CAZymes家族中,非专性寄生真菌的糖基水解酶家族GH和多糖裂解酶家族PL较专性寄生真菌显著扩增;功能聚类分析发现,非专性寄生真菌参与纤维素、果胶、木聚糖等植物细胞壁组分降解相关的基因较专性寄生真菌明显增多。结果表明了CAZymes与寄生类型的高度关联性,揭示了其在非专性寄生真菌致病过程中可能的关键作用。To understand the correlation between lifestyle and extracellular CAZymes, secretomes and CAZymes were predicted and annotated in phytopathogenic fungi, followed by comparative analysis. The results indicated that the ratio of secreted proteins to total putative proteins in hemibiotrophic and necrotrophic fungi was higher than that in biotrophic fungi. The CAZymes especially glycoside hydrolases （GH） and polysaccharide lyases （PL） subfamilies were expanded significantly in hemibiotrophic and necrotrophic fungi. Functional analysis of secreted CAZymes indicated that the plant cell wall （PCW） degrading enzymes involved in degradation of cellulose, pectin and xylan, were more abundant in hemibiotrophic and necrotrophic fungi. The distinct expansion of PCW degrading enzymes indicated the high correlation between fungal lifestyle and CAZymes, meanwhile revealing their potential roles in pathogenesis in hemibiotrophic and necrotrophic fungi.

关 键 词：植物病原真菌 寄生性 分泌蛋白组 碳水化合物酶类 植物细胞壁降解 

分 类 号：S432.4[农业科学—植物病理学]