基于缩硫醛脱保护机制的比例计量型Hg^(2+)荧光探针及其斑马鱼活体造影应用  

A novel ratiometric fluorescent probe for Hg^(2+) imaging in biological system

作　　者：陈韵聪[1,2] 朱成成[1,2] 何卫江[1,2] 郭子建[1,2] 

机构地区：[1]配位化学国家重点实验室 [2]南京大学化学化工学院,南京210093

出　　处：《中国科学：化学》2014年第4期631-638,636-638,共8页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(21271100;21131003和91213305);南京大学优秀博士研究生创新能力提升计划A资助(201301A05)

摘　　要：利用汞离子特异性诱导缩硫醛脱保护,引发分子内电荷转移发生改变的机制,设计合成了一种新型的激发型比例计量汞离子荧光探针.该探针在与汞离子结合后其最大激发波长由410 nm红移至485 nm,两个波长下的荧光强比值(F485/F410)由0.06增长至5.02.同时该探针对汞离子表现出了良好的比例计量响应选择性和较快的响应速度,并且检测限低于美国环境保护组织的饮用水标准.共聚焦造影研究发现,该探针可以应用于活细胞及5天龄斑马鱼幼体中的汞离子的检测.利用该性质对Hg2+在斑马鱼体内分布及毒性进行了初步的研究.A novel ratiometric fluorescent Hg2＋ probe, CDTT, was synthesized based on the changing of intramolecular charge transfer （ICT） process caused by the specific Hg2＋ triggered aldehyde recovery. A large excitation shift from 410 nm in the absence of Hg2~ to 485 nm in the presence of Hg2＋ was observed. The excitation intensity ratio of F48s/F41o increased from 0.06 to 5.02. CDTT exhibited high selectivity and fast response to Hg2＋, and its detection limit was determined to be 0.6 ppb, lower than the US EPA standard for drinking water. The ratiometric imaging ability in living cells and 5-day-old zebra fished was confirmed using laser scanning confocal fluorescent microscopy, the preliminary study of the distribution and the toxicity of Hg2＋ in zebra fish larvae were also conducted.

关 键 词：汞离子 荧光探针 比例计量 斑马鱼 

分 类 号：X835[环境科学与工程—环境工程] O657.3[理学—分析化学]