空间辨别性学习记忆活动对大鼠脑穹窿SDF-1免疫阳性细胞的影响  

Effect of spatial learning and memory activity on morphology of SDF-1positive cell cerebral fornix in rats

作　　者：李哲[1] 文秋嘉[2] 

机构地区：[1]广东医学院人体解剖学教研室,广东东莞523808 [2]广东医学院寄生虫教研室,广东东莞523808

出　　处：《广州医学院学报》2013年第5期5-7,共3页Academic Journal of Guangzhou Medical College

摘　　要：目的:探讨大鼠空间辨别性学习记忆活动大鼠脑穹窿基质细胞衍生因子-1(SDF-1)免疫阳性细胞表达的影响。方法:用水迷宫训练SD大鼠建立空间辨别性学习记忆模型,用免疫组化方法和图像分析技术检测大鼠脑穹窿SDF-1免疫阳性细胞的形态变化。结果:对照组大鼠脑穹窿可见少量圆形或椭圆形SDF-1免疫阳性细胞分布。免疫阳性细胞的胞浆呈淡黄色,颗粒状免疫反应产物附于细胞膜内面。游水组大鼠脑穹窿SDF-1免疫阳性细胞的分布及形态与对照组相比均未见明显差异;模型组训练7 d的大鼠脑穹窿SDF-1免疫阳性细胞的形态和分布与对照组相比未见明显差异。训练14 d和21 d的大鼠神经元细胞在形态上以锥体形或三角形为主。在SDF-1免疫阳性细胞的数量表达上,训练21 d的比训练7d和14 d的明显增多。结论:大鼠脑穹窿内SDF-1免疫阳性细胞可能参与空间辨别性学习记忆活动引致的形态学可塑。Objective： To explore effects of the activity of spatial learning and memory on cell morphology of SDF-1 immuno-positived and SDF-I expression in cerebral fornix in rats. Methods： spatial learning and memory activities of SD male rats were trained with water maze. Immunohistochemistry and imaging analyzing system were used to study the location, shape and number changes of SDF-l-positive cells in rat cerebral fornix. Results：The positive cells of SDF-limmunoreactive in cerebral fornix distributed . Which the SDF-l-positive cells in rats cerebral fornix that the swimming group compared with the control group, there is no significant difference about the cell morphology and distribution Which the SDF-i-positive ceils in rats cerebral fornix that the model rats trained 7 days compared with the control group, there is no significant difference about the cell morphology and distribution. Morphology of the rats neuron cells was mainly pyramid and triangular which of the model trained 14 days and 21 days. In the number expression of SDF-l-positive ceils, training 21 d increased significantly more than training for 7 d and 14 d. Conclusion ： SDF-1 maybe correlates with the spatial learning and memory activity and maybe take part in the cellular morphological plasticity in cerebral fornix.

关 键 词：基质细胞衍生因子-1 脑穹窿 空间辨别性学习记忆 

分 类 号：R332[医药卫生—人体生理学]