天灸对哮喘模型大鼠细胞因子及特异性转录因子的影响  

Effects of natural moxibustion on cytokines and specific transcription factors in asthma rats

作　　者：李月梅[1] 凌翠敏 

机构地区：[1]广州中医药大学第一附属医院针灸科,广东广州510405

出　　处：《中国针灸》2014年第4期379-383,共5页Chinese Acupuncture & Moxibustion

基　　金：广东省自然科学基金项目:S 201101000562

摘　　要：目的:探讨天灸调节哮喘大鼠免疫失衡的机制。方法:将70只SD雄性大鼠分为正常组、安慰剂组、地塞米松组、大饼长程贴药组、大饼短程贴药组、小饼长程贴药组、小饼短程贴药组,每组各10只,除正常组外均采用卵白蛋白致敏和激发建立大鼠哮喘模型,对贴药组进行天灸治疗,其中大饼贴药组用1cm×1cm的大药饼贴药,小饼贴药组用0.5cm×0.5cm的小药饼贴药,每次按照相关穴位进行贴药,每次贴药5h,每天1次,共4次为一疗程。长疗程组共治疗3个疗程,短疗程组治疗1个疗程。地塞米松组予地塞米松进行腹腔注射,疗程与长程贴药组相同。实验结束后,观察哮喘大鼠外周血中嗜酸性粒细胞(EOS)变化;用酶联免疫吸附法(ELISA)检测大鼠肺组织中免疫球蛋白E(IgE)、细胞因子白介素4(IL-4)及干扰素γ(IFN-γ)的含量变化;用实时荧光定量(real-time Q-PCR)技术检测肺组织转录因子T-bet(T-box express in T cell)及GATA-3(GATA binding protein 3)蛋白的表达水平。结果:与正常组比较,安慰剂组全血EOS计数及血清IL-4、IgE含量均升高(均P<0.01),血清中IFN-γ含量明显降低(P<0.01));天灸贴药各组及地塞米松组可显著降低哮喘大鼠EOS、IgE水平及IL-4含量(均P<0.01),提高IFN-γ的含量(P<0.01)。与正常组比较,安慰剂组中T-bet mRNA表达显著降低(P<0.01);各治疗组能显著提高T-bet mRNA的表达,明显减少GATA-3mRNA的表达(P<0.01)。与短程贴药组比较,长程贴药组及地塞米松组中T-betmRNA的表达明显升高(P<0.01),GATA-3mRNA的表达显著降低(P<0.01);长程贴药组与地塞米松组比较无显著性差异(P>0.05),大饼贴药组与小饼贴药组比较亦无显著性差异(P>0.05)。结论:天灸能明显减轻哮喘大鼠的气道炎症,随时间的延长,疗效增加,具有明显的时效性,与药饼的大小无明显关系。asthma rats. Objective To explore the mechanism of natural moxibustion on regulating immune imbalance in Methods Seventy SD male rats were divided into a normal group, a placebo group, a dexamethasone group, a big-cake for long-course moxibustion group, a big-cake for short-course moxibustion group, a small-cake for long-course moxibustion group and a small-cake for short-course moxibustion group, ten rats in each one. The rat model of asthma was established by egg albumen sensitization and stimulation in all the groups except the nor- mal group. The natural moxibustion was used in all moxibustion groups, in which big cake of 1cm×1cm size was used in the big-cake groups and small cake of 0.5 cm× 0.5 cm size was used in the small cake groups. According to relevant acupoints, the natural moxibustion was performed, 5 h per time, once a day. Four times of treatment was considered one course, and three courses were required in the long-course groups and one course was required in the short-course groups. Intraperitoneal injection of dexamethasone was applied in the dexamethasone group, which had the same course as long-course moxibustion group. After the treatment, changes of EOS in peripheral blood of asthma rats were observed; enzyme linked immunosorbent assay （ELISA） was applied to test the contents of IgE, IL-4 and IFN-γ in the lung tissue; real-time Q-PCR method was adopted to measure the expression level of transcription factor T-bet and GATA binding protein 3 （GATA-3） in the lung tissue. Results Compared with the normal group, the EOS in whole blood as well as IL-4 and IgE in plasma were all increased in the placebo group （all P〈 0.01）, IFN-γ in plasma was obviously decreased （P〈0.01）; while the levels of EOS, IgE and IL-4 were signifi- cantly reduced （all P〈0.01）, the content of IFN-γ was increased （P〈0.01） in all moxibustion groups and dexa- metnasone group. Compared with the normal group, the expression of T-bet mRNA in the placebo group was sig- nificantly reduced （P�

关 键 词：哮喘大鼠 天灸 细胞因子 转录因子 量效 时效 

分 类 号：R245.8[医药卫生—针灸推拿学]