脑微透析技术在胶质瘤研究中的应用进展  

Research progress in the study of brain microdialysis in glioma

作　　者：柳琳[1] 张相宜[1] 肖瑶[2] 张幸国[1] 

机构地区：[1]浙江大学医学院附属第一医院药剂科,浙江杭州310003 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《药学学报》2014年第4期450-456,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81072584)

摘　　要：胶质瘤是最常见的颅内肿瘤,目前尚无有效的治疗方法。深入了解胶质瘤的生理代谢特征以及化疗药物在脑内的药代动力学过程,对改善胶质瘤的治疗具有重要意义。脑微透析(brain microdialysis,B-MD)技术为中枢神经系统、生理和药理研究提供了有效手段,是目前在生理和病理情况下研究活体药物脑内分布、药物透过血脑屏障情况及监测脑内药物有效活性物质和神经递质变化的有效方法,有望为临床设计胶质瘤的个体化化疗方案提供参考依据,也为评价新型抗肿瘤药物的体内过程提供有力工具。本文将对B-MD的特点、探针的埋植及其与其他技术的比较进行归纳总结,并概述其在胶质瘤的代谢产物、药动学研究、神经递质等方面的研究进展,以了解脑微透析技术在胶质瘤研究中的应用概况。Glioma is the most common form of brain cancer. Despite recent advances in the treatment of solid tumors, there are few effective treatments for malignant gliomas due to its infiltrative nature. It has important significance to improve the treatment of glioma through in-depth understanding the intracerebral metabolic characteristics and pharmacokinetics of chemotherapeutics. Brain microdialysis （B-MD）, an effective method to monitor central nervous system anticancer drug disposition, conditions of drugs through the blood- brain barrier, basic pathophysiologic metabolism, bioactive compounds and the changes of neurotransmitter in brain, provides the unique opportunity to allow the simultaneous determination of unbound concentrations of drugs in several tissues, and directly measure gliomas biochemistry continuously. B-MD has been able to monitor the change of brain drugs, metabolites and neurotransmitters, dynamic analysis of the drug concentration and pharmacological effect after administration, pharmacodynamic interaction between drugs, receptor mechanism of drug transport, as well as feedback information of internal environment. B-MD is expected to provide reference for clinical individual chemotherapy of glioma, but also provide powerful tools for the evaluation of new anticancer drugs in vivo. In this review, a comprehensive overview of B-MD for studies on glioma is elucidated with special emphasis on its application to neurochemistry and pharmacokinetic studies.

关 键 词：微透析 胶质瘤 神经递质 药动学 代谢 

分 类 号：R917[医药卫生—药物分析学]