FGF-21对H_2O_2诱导的大鼠心肌细胞H9c2氧化应激损伤的保护作用  

FGF-21 protects H9c2 cardiomyoblasts against hydrogen peroxide-induced oxidative stress injury

作　　者：韩苗苗[1] 王文飞[1] 刘铭瑶[1] 李德山[1] 周兵[1] 于引航[1] 任桂萍[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《药学学报》2014年第4期470-475,共6页Acta Pharmaceutica Sinica

基　　金：黑龙江省教育厅科学技术研究项目(12521z004);国家自然科学基金基础人才培养项目(J1210069-31)

摘　　要：成纤维细胞生长因子-21(fibroblast growth factor-21,FGF-21)是机体内重要的代谢调节因子,但尚不清楚FGF-21对心血管系统的直接影响。本研究利用H2O2诱导的心肌细胞H9c2氧化应激模型,分别用MTT、流式细胞术、real-time PCR等方法评价FGF-21是否具有对抗氧化损伤引起的心肌细胞凋亡的能力及其相关作用机制。结果显示,FGF-21能提高H2O2处理后的H9c2细胞存活率,降低心肌细胞凋亡,并减少H2O2引起的ROS累积;而且FGF-21通过上调SOD活性以及调控Bcl-2/Bax的表达量,发挥抑制细胞凋亡的作用。研究结果证实,FGF-21能够保护心肌细胞免受氧化应激带来的损伤。Fibroblast growth factor-21 （FGF-21） is an important metabolism regulator, however, whether FGF-21 has effects on cardiovascular remains unclear. In this study, H2O2-induced injury in H9c2 cells was used as a cell model, the anti-apoptosis potential and mechanism of FGF-21 against oxidative injury were evaluated by MTT assay, flow cytometry assay and real-time PCR. The results showed that FGF-21 could increase the cell survival of H2O2-induced injury in H9c2 cells and prevent H9c2 cells from oxidative stress- induced apoptosis. Furthermore, FGF-21 can elevate SOD activity and regulate Bcl-2/Bax expression in H9c2 cells. The results suggest that FGF-21 have protective effect against the H2O2-induced apoptosis in H9c2 cells.

关 键 词：FGF-21 心肌细胞 过氧化氢 氧化应激损伤 细胞凋亡 

分 类 号：R963[医药卫生—微生物与生化药学]