适用于分离人肠道中双歧杆菌的选择性培养基  

Selective medium to isolate human Bifidobacterium

作　　者：霍冬雪[1] 张家超[1] 白娜[1] 黄卫强[1] 张和平[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特010018

出　　处：《微生物学报》2014年第4期433-441,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金项目(30660135)~~

摘　　要：【目的】比较并评价5种双歧杆菌选择性培养基对人源双歧杆菌的分离效果,试图筛选出一种适用于人肠道中双歧杆菌分离培养的选择性培养基。【方法】采集6份健康人粪便样品稀释涂布于5种选择性培养基上,厌氧培养后计数菌落并挑选单菌落进行鉴定。同时提取样品中细菌宏基因组DNA,应用变性梯度凝胶电泳技术(Denaturing Gel Gradient Electrophoresis,DGGE)和荧光定量PCR技术(Quantitative Polymerase Chain Reaction,q-PCR)揭示样品中双歧杆菌种类和数量,并以此为依据,客观评价上述5种选择性培养基的分离效果。【结果】BSM培养基和BLM培养基上双歧杆菌的计数结果与q-PCR的定量结果最为接近,并显著高于其它3种培养基。BLM培养基上分离到双歧杆菌的种类与DGGE图谱多样性分析的结果最为接近。【结论】BLM培养基是一种适用于人肠道中双歧杆菌分离培养的选择性培养基。[ Objective ] To compare five selective media to isolate human Bifidobacterium [ Methods ] Feces from six healthy human volunteers were diluted and cultivated on five Bifidobacterium selective media. After anaerobic cultivation, bacterial colonies were counted, selected and identified. Meanwhile, bacterial genomic DNA was extracted from the feces samples, and the Denaturing Gradient Gel Electrophoresis （DGGE） and Quantitative Polymerase Chain Reaction （q- PCR） were applied to reveal the diversity of Bifidobacterium. [ Results] The amount of Bifidobacterium grown on BSM and BLM media was similar to the result detected by q-PCR and was significantly higher than that on three other media. Bifidobacterium isolated from BLM medium was similar to the identified result of DGGE profile. [ Conclusion] BLM medium is the best selective medium for Bifidobacterium isolation from human gastrointestinal tract.

关 键 词：双歧杆菌 变性梯度凝胶电泳技术 荧光定量PCR技术 选择性培养 

分 类 号：R378[医药卫生—病原生物学]