2型糖尿病患者尿上清microRNA-29水平与尿白蛋白水平的相关性  

Urinary microRNA- 29 correlates with urinary albumin in type 2 diabetes mellitus

作　　者：彭晖 钟美容 赵文波 王成 张俊 刘迅 李远清 SujayDuttaPaudel 王倩倩 娄探奇 

机构地区：[1]中山大学附属第三医院肾内科,广州510630

出　　处：《中华肾脏病杂志》2014年第4期262-266,共5页Chinese Journal of Nephrology

基　　金：国家自然科学基金（30800408,30771011,81170678）;中央高校基本科研业务费专项资金（10ykpy03）;“十二五”国家科技支撑计划（2011BAI10800）

摘　　要：目的探讨2型糖尿病患者尿上清的microRNA-29（miR-29）水平作为糖尿病肾病的生物标志物的可能性。方法收集61例2型糖尿病患者的清晨首次中段尿，根据尿白蛋白水平分为糖尿病无蛋白尿组m=25，（58．88±11．75）岁]和糖尿病有蛋白尿组In=36，（62．19±13．11）岁1。用实时荧光定量PCR法检测尿上清miR-29a、miR-29b和miR-29c的水平。等量的外源性人工合成的cel．miR．39加入等体积的尿上清中用于校正miR-29的水平。同时收集尿白蛋白排泄率、血肌酐、尿素氮、糖化血红蛋白（HbAlc）、血脂等其他生化指标。而眼底检查的结果则作为评估糖尿病小血管病变的指标。结果2型糖尿病有、无蛋白尿两组之间HbAlc水平及糖尿病病程差异无统计学意义，而2型糖尿病有蛋白尿组估算肾小球滤过率（eGRF）水平显著低于无蛋白尿组（P=0．001），2型糖尿病有蛋白尿组尿上清miR-29a、miR-29b和miR-29c水平高于无蛋白尿组（P=0．029、0．032、0．040）。2型糖尿病尿白蛋白排泄率与尿上清miR．29a及miR-29b水平相关（r=0．284，P=0．039；r=0．275，P=0．046），尿上清miR．29b水平与尿素氮水平相关（r=0．277，P=0．031），而miR．29水平与上述其他各项临床指标无相关。结论2型糖尿病患者尿上清miR-29a及miR．29b水平与尿白蛋白水平相关。尿上清miR-29作为2型糖尿病患者糖尿病肾病的生物标志物的潜能有待进一步的探索和评估。Objective To investigate the possibility of urinary mieroRNA- 29 （miR- 29） as biomarker for diabetic nephropathy in patients with type 2 diabetes. Methods Sixty one patients with type 2 diabetes were divided into 2 groups： diabetes with normoalbuminnria[n = 25, （58.88±11.75） years oldland diabetes with albuminuria[n = 36, （62.19± 13.11） years old]. There were no significant differences in age and gender between groups. The contents of miR-29a, miR- 29b and miR- 29c in urine supematant were determined by real- time quantitative PCR, and a synthetic cel- miR- 39 was added to the urine as a spike-in control before miRNAs extraction. The laboratory parameters including urinary albumin excretion, serum creatinine, BUN, glycosylated hemoglobin, and blood lipids were collected, while retinopathy serves as non-invasive method to assess vascular fibrosis. Results There was no significant difference in glycosylated hemoglobin levels and duration of diabetes between twogroups, while the diabetes with albuminuria group had lower estimated glomerular filtration rate （eGFR） （P= 0.001）, had higher level of nfiR-29a, miR-29b and miR-29c in urine （P = 0.029, 0.032, 0.040） compared with diabetes normoalbuminuria group. Urinary albumin excretion rate significantly correlated with urinary miR-29a level （r = 0.284, P = 0.039） and miR-29b level （r = 0.275, P = 0.046）, urinary miR- 29b significantly correlated with BUN （r = 0.277, P = 0.031 ）in patients with type 2 diabetes. However, no correlation was found between miR-29a, miR-29b, miR-29c and other clinical parameters. Conclusion Urinary miR-29a and miR-29b correlates with urinary albumin in patients with type 2 diabetes, and it needs further exploration and evaluation for urinary miR- 29 to serve as potential biomarker for diabetic nephropathy in type 2 diabetes.

关 键 词：微RNAS 糖尿病肾病 生物学标记 microRNA-29 

分 类 号：R587.1[医药卫生—内分泌]