邻苯二甲酸二(2-乙基己基)酯对调节性T细胞分化和功能的影响  

The effects of Di-( 2-ethylhexyl) phthalate on regulatory T cells differentiation and function

作　　者：韩毓[1] 陆颖[2] 朱文俊[3] 胡培培[3] 徐广飞[2] 

机构地区：[1]南通大学公共卫生学院职业医学与环境卫生学系,江苏南通226019 [2]南通大学公共卫生学院营养与食品卫生学系 [3]南通大学医学院免疫学系

出　　处：《毒理学杂志》2014年第1期26-29,共4页Journal of Toxicology

基　　金：南通市科技计划项目(BK2011009;BK2011049)

摘　　要：目的研究环境持久性有机污染物邻苯二甲酸二(2-乙基己基)酯(Di-(2-ethylhexyl)phthalate,DEHP)对调节性T细胞(regulatory T cells,Treg)分化比例及其抑制功能的影响。方法构建生长期小鼠DEHP暴露模型,采用流式细胞术检测脾脏CD4+CD25+Foxp3+Treg细胞数量;免疫磁珠分选法制备CD4+CD25+T细胞和CD4+CD25-T细胞,CCK法检测CD4+CD25+T细胞和CD4+CD25-T细胞增殖能力,混合淋巴细胞反应检测CD4+CD25+T细胞对CD4+CD25-T细胞增殖的抑制作用。结果生长期小鼠经DEHP暴露处理后,CD4+CD25+Foxp3+Treg细胞数量逐渐减少,CD4+CD25+T细胞体外增殖能力及其抑制CD4+CD25-T细胞增殖的作用均逐渐降低。结论 DEHP能够从数量和功能两方面下调CD4+CD25+Treg细胞分化比例及其对效应性细胞的抑制功能。Objective To study the effects of persistent organic pollutant Di-（2-ethylhexyl）phthalate （DEHP） on regulatory T cells （Treg） differentiation rate and inhibition function. Methods Micespleen CD4^＋ CD25^＋ Foxp3^＋ Treg cells quantity was evaluated using flow cytometry at the growing mice model of DEHP exposure. CD4 ^＋ CD25 ^＋ T cells and CD4^＋ CD25^ - T cells were isolated and purified with immunomagnetic beads. The proliferation of CD4 ^＋ CD25^＋ T cells and CD4 ＋ CD25^ - Tcells were detected using CCK assay. The inhibition of CD4 ^＋ CD25^＋ Tcells on CD4^＋ CD25^ - T cells proliferation were analyzed using mixed lymphocyte reaction. Results After the treatment of DEHP exposure, growing micespleen CD4^＋ CD25 ^＋ Foxp3^＋ Treg cells quantity, CD4^＋ CD25^＋ T cells proliferation and their inhibition on CD4^＋ CD25^- T cells proliferation in vitro decreased gradually. Conclusion DEHP may reduce CD4^＋ CD25^＋ Treg ceils differentiation rate and their inhibition function on effector cells.

关 键 词：邻苯二甲酸二(2-乙基己基)酯 调节性T细胞 免疫抑制 

分 类 号：R114[医药卫生—卫生毒理学]