巨大芽胞杆菌B196菌株抑菌物质的分离纯化  

Isolation and purification of an antimicrobial substance produced by Bacillus megateriumstrain B196

作　　者：廖庭[1] 秦健[1] 袁高庆[1] 黎起秦[1] 林纬[1] 彭好文[1] 

机构地区：[1]广西大学农学院,南宁530005

出　　处：《植物保护》2014年第2期16-21,27,共7页Plant Protection

基　　金：广西自然科学基金项目(2011GXNSFA018064)

摘　　要：巨大芽胞杆菌(Bacillus megaterium)B196菌株产生的拮抗物质对水稻纹枯病菌(Rhizoctonia solani)的生长具有较强的抑制作用,明确该菌产生拮抗物质的种类是进一步研究该菌的抑菌机制及其应用的基础。本文采用盐酸沉淀B196菌株的去菌体培养液,再用甲醇抽提获得拮抗物质的粗提物。利用反相HPLC系统,将粗提物过C18柱,收集有抑制水稻纹枯病菌生长作用的活性化合物。运用质谱测得其分子量分别为1 042.592 7u,再利用碰撞诱导解离(CID)技术获得化合物的典型结构特征离子碎片,结果表明其一级结构为Pro-Asn-Ser-βAA-Asn-Tyr-AsnGln(βAA为14个碳原子的氨基脂肪酸)。综合以上信息将该化合物鉴定为Iturin A2。An antimicrobial substance produced by Bacillus megaterium strain B196 can strongly inhibit the growth of Rhizoctonia solani.Identification of the antimicrobial substance is very important for further research on the inhibition mechanism and its application.Crude antimicrobial substances were extracted with methanol from the precipitate which was obtained by adding HC1 to the cell-free culture broth and then stored at 4 ℃ overnight.The crude extract was further purified by reversed-phase HPLC with a C18 column（PRC-ODS,20 × 250 nm）to separate the substances.An antifungal compound with strong inhibitory activity against R.solani was purified.The molecular weight of the compound was 1 042.592 7 u by Matrix-assisted laser desorption ionization time-of-flight mass spectrometer（MALDI-TOF-MS）.TESI collision induced dissociation mass spectrometry analysis demonstrated that the compound had a primary structure of Pro-Asn-Ser-βAA-Asn-Tyr-Asn-Gln.Therefore,the compound was identified as Iturin A2.

关 键 词：巨大芽胞杆菌 ITURIN A2 分离纯化 

分 类 号：S482.292[农业科学—农药学]