Cry1Ba3蛋白对亚洲玉米螟杀虫活性测定及其与BBMVs的结合分析  

Analysis of insecticidal activity of Cry1Ba3 protein against Ostrinia furnacalis and binding assay with BBMVs

作　　者：尹凤翔 孙凯[1] 束长龙[1] 宋福平[1] 张杰[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护》2014年第2期22-27,共6页Plant Protection

基　　金：国家"973"计划项目(2009CB118902);国家自然科学基金项目(31272115)

摘　　要：提取并纯化Cry1Ba3蛋白截短片段,用饲喂人工饲料的方法对亚洲玉米螟（Ostrinia furnacalis）初孵幼虫进行生物活性测定.结果显示,Cry1Ba3截短片段对亚洲玉米螟有良好的杀虫活性（LC50=5.29 μg/g,95％置信限为3.96～7.10 μg/g）.而纯化的Cry1Ac蛋白60 ku的活性片段对亚洲玉米螟初孵幼虫的杀虫活性LC50为0.83 μg/g.提取亚洲玉米螟5龄幼虫的BBMVs,用纯化的Cry1Ba3和Cry1Ac蛋白进行竞争结合试验,结果显示Cry1Ba3与Cry1Ac在亚洲玉米螟中肠的结合没有竞争作用,说明两种毒素在亚洲玉米螟BBMVs上可能存在不同的受体.Truncated fragment of Cry1Ba3 protein was extracted and purified.Bioassay against neonates of Ostrinia furnacalis was conducted by feeding artificial diet with the truncated fragment of Cry1Ba3.The result showed truncated fragment of Cry1Ba3 had a high toxicity against O.furnacalis（LC50 =5.29 μg/g,95％ fiducial limit was between 3.96 and 7.10 μg/g）.LC50 of purified active fragment of Cry1Ac protein,with molecular weight of 60 ku,was 0.83μg/g against neonates of O.furnacalis.Then the BBMVs of the fifth instar larvae of O.furnacalis was extracted.Competitive binding experiment was conducted by using purified Cry1Ba3 and Cry1Ac protein.The result showed Cry1Ba3 and Cry1Ac had no competitive action in the process of binding to midgut of O.furnacalis,indicating these two toxins may have different receptors on BBMVs of O.furnacalis.

关 键 词：Cry1Ba3蛋白 Cry1Ac蛋白 亚洲玉米螟 竞争结合 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]