云南省玉米灰斑病菌的分子鉴定  

Molecular identification of maize grey leaf spot pathogens in Yunnan Province

作　　者：黄惠川 杨敏[1] 王岩[2] 何霞红[1] 李成云[1] 朱有勇[1] 朱书生[1] 

机构地区：[1]云南农业大学教育部农业生物多样性与病害控制重点实验室,昆明650201 [2]吉林大学农学部植物科学学院,长春130012

出　　处：《植物保护》2014年第2期28-35,共8页Plant Protection

基　　金：国家"973"计划项目(2011CB100400)

摘　　要：本研究利用ITS区、tef1基因、cmdA基因和HIS基因4个基因部分序列对2009-2011年间采自云南省主要灰斑病发生区域和吉林省部分区域的玉米灰斑病菌进行比较分析。结果表明,无论是4个基因序列单独聚类还是拼接序列聚类均把云南省灰斑病发生区域采集的菌株与Cercospora zeina聚为一群,而吉林省采集的灰斑病菌则与Cercospora zeae-maydis聚为一群。聚类分析还表明云南省不同玉米产区的灰斑病菌遗传背景单一,在所有聚类树中均聚为一群,且自举支持值均>99%。Monoconidial isolates of the fungus causing gray leaf spot of maize were obtained from diseased leaves collected throughout the Yunnan Province and part area of Jilin Province and analyzed for genetic variability with the method of cluster analysis.The DNA sequences for the internal transcribed spacers （ITS1 ＆ ITS2）,the 5.8S rRNA gene,elongation factor 1-α,histone H3 and calmodulin gene regions suggest two very distinct groups.Both groups were found to be relatively uniform internally with an average genetic similarity among isolates of approximately 99％.Isolates from Yunnan were always be in the same clade with Cercospora zeina,and isolates from Jilin always with C.zeae-maydis,regardless of analyses with 4 single gene date matrix respectively or with combined data matrix.

关 键 词：玉米灰斑病 分子鉴定 基因序列比对 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]