基于CCD的阵列毛细管电泳芯片检测系统研究  

Study on Fluorescence Detection System for Capillary Array Electrophoresis Microchip Based on CCD

作　　者：杨晓博[1] 闫卫平[2] 杨飞[1] 窦伟峰[2] 

机构地区：[1]许昌学院电气信息工程学院,河南许昌461000 [2]大连理工大学电子科学与技术学院,辽宁大连116024

出　　处：《仪表技术与传感器》2014年第3期61-64,共4页Instrument Technique and Sensor

基　　金：河南省教育厅科学技术研究重点项目(13B510240)

摘　　要：阵列毛细管电泳芯片检测系统的性能决定其并行检测的精度及可靠性。搭建出一种以CCD(TCD1304AP)为光电转换器件的阵列毛细管电泳芯片荧光检测系统,系统以LED为激发光源,可以同时检测4个通道内样品经分离后受光源激发所产生的荧光信号。系统光路部分利用LED光源、滤光片及针孔的有效组合,简化了光路结构;CCD器件将荧光信号转换为模拟信号,通过高速A/D转换后经USB接口发送至上位机进行实时显示。系统对不同浓度的Rhodamine B样品溶液进行了电泳分离实验,结果表明系统具有结构简单、实时性强、灵敏度较高等优点。The accuracy and feasibility of parallel detection for capillary array electrophoresis microchip is determined by the performance of fluorescence detection system.A fluorescence detection system was presented in which CCD （TCD1304AP） and LED were used as photoelectric device and light source,respectively.The analog signals were transformed to digital signals by highspeed A/D device and then were sent to host computer for real-time display.Different concentrations of Rhodamine B sample solution were introduced into the microchannels for electrophoretic separation and the results showed that the system had advantages of compact structure,real-time process,and higher sensitivity

关 键 词：TCD1304AP 毛细管电泳芯片 荧光检测 LED光源 电泳分离 

分 类 号：TP273[自动化与计算机技术—检测技术与自动化装置]