HPLC法测定肠虫清胶囊中阿苯达唑的含量  

Determination of albendazole in changchongqing capsules by HPLC

作　　者：游国叶[1] 熊大伟[1] 

机构地区：[1]信阳职业技术学院药学院,河南信阳464000

出　　处：《河南大学学报（医学版）》2014年第1期33-35,共3页Journal of Henan University:Medical Science

基　　金：河南省自然科学基金项目(052442004)

摘　　要：目的建立HPLC法测定肠虫清胶囊中阿苯达唑的含量。方法采用Luna C18（5μm,250×4.6mm）柱,以甲醇-0.1mol/L醋酸铵缓冲液（用醋酸调节pH至3.1）（60∶40）为流动相,检测波长288nm。结果阿苯达唑的线性范围为0.1-1.0mg/mL,回归方程为：Y=2.85×106 X＋6.5×104（r=0.999 5）;平均回收率为99.85%,RSD为0.33%。结论本法快速、准确,结果可靠,重现性好,适于肠虫清胶囊的质量控制。Objective To determine the albendazole in changchongqing capsules by HPLC. Methods Luna C18 （5μm, 250× 4.6 mm） column was used, methanol- 0.1 mol/L ammonium acetate buffer （pH ： 3.1 ） （60 ： 40） served as mobile phase . The wavelength for detection was 288 nm. Results The linear range of albendazole was 0.1 - 1.0 mg/mL and the regression equation of albendazole was Y ： 2.85 × 10^6 X＋ 6.5 × 10^4 （r = 0. 999 5）; The average recovery was 99.85 % （ RSD = 0.33 %）. Conclusion The method is sensitive, accurate, reliable, reproducible and suitable for quality control of changchongqing capsules.

关 键 词：HPLC 肠虫清胶囊 阿苯达唑 含量测定 

分 类 号：R927.2[医药卫生—药学]