抗人DR5抗体mDRA-6诱导EC109细胞自噬和凋亡  

EC109 cells autophagy and apoptosis induced by anti-human death receptor 5 monoclonal antibody( mDRA-6)

作　　者：贾彩云[1] 赵粤萍[1] 刘广超[1] 马远方[1] 

机构地区：[1]河南大学细胞与分子免疫学重点实验室,开封475000

出　　处：《中国免疫学杂志》2014年第3期303-306,322,共5页Chinese Journal of Immunology

基　　金：国家"重大新药创制"科技重大专项资助(No.2011ZX09506-004)

摘　　要：目的：探讨鼠抗人D1L5单克隆抗体（mAb）mDRA-6对ECl09细胞自噬和凋亡诱导作用。方法：MTY法检测mDRA-6细胞毒性；MDC染色和Hoechst33258染色，观察mDRA-6诱导细胞自噬、凋亡形态学变化；流式细胞术定量分析mDRA-6诱导细胞自噬率和凋亡率。结果：mDRA-6具有细胞毒性，0．024～25mg／LmDRA-6作用细胞呈时间剂量依赖性．各组间差异具有统计学意义（P〈0．01），10h的IC50值为0．78mg／L；经mDRA-6处理ECl09细胞出现核固缩、染色质边缘化和形成凋亡小体等；同时细胞浆中出现自噬体；流式细胞术测定结果显示，2．0mg／LmDRA-6作用细胞10h，细胞自噬率和凋亡率分别为19．44％和54．88％。结论：mDRA-6可引起ECl09细胞自噬和凋亡；mDRA-6通过诱导ECl09细胞自噬和凋亡抑制细胞生长。Objective：To investigate the apopototic action and its autophatic action of a novel anti-human DR5 monoclonal anti- body mDRA-6 on EC109 cells. Methods： The cytotoxic action of mDRA-6 on EC109 cells were detected by MTT assay. And the cells morphologic changes were observed by fluorescence microscopy with Hoeches 33258 staining or MDC staining. And MDC positive cells ratio and the apoptotic ratio induced by mDRA-6 were measured by flowcytometic analysis. Results： 0.024 - 25 mg/L mDRA-6 exer- ted cytotoxicity on EC109 cells in dose-dependent and time-dependent manner. Differences between groups were statistically significant. The ICs0 was approximately 0.78 p.g/ml after treating with mDRA-6 for 10 h. mDRA-6 induced the formation of caryopycnosis, chro- matic marginalization,apoptotic bodys and increases of MDC recuritment in EC109 cells at the same time. Flowcytometric analysis showed increasing autophatic ratio and apoptotic ratio with 2.0 mg/L mDRA-6 on EC109 cells for 10 hours. Conclusion： mDRA-6 may induce EC109 cells autophagy and apoptosis, mDRA-6 exerts cytotoxic effect on EC109 cells by the inducation of autophagy and apoptosis.

关 键 词：抗DR5单克隆抗体(mDRA6) EC109细胞 自噬 凋亡 

分 类 号：R392.1[医药卫生—免疫学]