云芝糖肽对EAC荷瘤小鼠脾脏TLR4信号通路调控机制的研究  

Immunomodulatory effects of polysaccharopeptide on spleen of EAC tumorbearing mice through TLR4 signaling pathway

作　　者：董冰[1] 冯子芳[1] 余爽[1] 鲍依稀[1] 

机构地区：[1]重庆医科大学附属第二医院检验科,重庆400010

出　　处：《中国免疫学杂志》2014年第3期333-337,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81274144);重庆市教委课题项目(KJ110303);重庆市卫生局中医药科技项目(2010-2-147)资助

摘　　要：目的:研究云芝糖肽(PSP)对荷瘤小鼠脾脏TLR4信号通路的调控作用,探讨PSP在体内的免疫调节及信号转导机制。方法:建立艾氏腹水癌(EAC)实体型荷瘤小鼠模型,随机分为2组:生理盐水组(NS组)、云芝糖肽组(PSP组),灌胃给药30 d后,采用实时荧光定量PCR(Q-PCR)检测两组小鼠脾脏中TLR4通路相关基因的表达水平,采用免疫印迹法(Western blot)检测信号通路相关蛋白的表达情况。结果:与NS组相比,PSP组荷瘤小鼠脾脏TLR4信号通路中的TLR4、IRAK4、TRAF6、TAK1、IKKα、NF-κB、JNK、ERK、P38、AP-1基因的相对表达量均明显提高(P<0.05),通路中的相关蛋白TLR4、TRAF6、NF-κB和AP-1均明显上调。结论:云芝糖肽在体内对荷瘤小鼠的免疫调节作用可能是通过TLR4信号通路来实现的。Objective：To study the effects of polysaceharopeptide （PSP） on TLR4 signaling pathway in the spleens of EAC tumor-bearing mice, and explore the immunomodulatory mechanism of PSP. Methods ： Ehrlich＇s ascites carcinoma （EAC） Kunming （KM） mice were used to establish the animal model for solid tumor. Mice were randomly divided into two groups： NS group and PSP group. After 30 days of treatment, quantitative real-time PCR （Q-PCR） and Western blot were used to detect the related gene and pro- tein expression of TLR4 pathway in spleens from each group. Results： Compared with NS group, genes in TLR4 pathway was up-regu- lated in the PSP group： TLR4, IRAK4, TRAF6, TAK1, IKKα, NF-kB, JNK, ERK, P38 and AP-1 （P 〈 0.05）. Meanwhile, the re- lated proteins of TLR4 pathway （ TLR4, TRAF6, NF-kB and AP-1 ） were up-regulated in PSP group（ P 〈 0.05 ）. Conclusion ： The im- munoregulatory effect of PSP on EAC tumor-bearing mice may be implement through the regulation of TLR4 signaling pathway.

关 键 词：云芝糖肽 TLR4 艾氏腹水癌 免疫调节 

分 类 号：R285.5[医药卫生—中药学] R392.5[医药卫生—中医学]