Sema 4D基因在原发免疫性血小板减少症的表达及与T辅助细胞因子关系  

Sema 4D genes expressed in primary immune thrombocytopenia and relationship with T helper cells factor

作　　者：薛乾富[1] 肖悦[1] 

机构地区：[1]重庆医科大学附属永川医院血液内科,重庆402160

出　　处：《中国免疫学杂志》2014年第3期400-403,共4页Chinese Journal of Immunology

摘　　要：目的:探讨Sema 4D基因在原发免疫性血小板减少症的表达及与T辅助细胞因子关系。方法:分别采用实时定量聚合酶链反应和微量样本多指标流式蛋白定量技术(CBA)分别检测外周血单核细胞Sema 4D mRNA水平和Th1/Th2细胞因子水平,分析Sema 4D mRNA水平和Th细胞因子水平的相关性分析。结果:(1)ITP患者外周血单核细胞Sema 4D mRNA水平显著高于健康对照志愿者(P<0.01),外周血清Th1细胞因子IL-2和IFN-γ水平显著低于健康对照受试者(P<0.05或P<0.01),Th2细胞因子IL-4、IL-6和IL-10水平显著高于健康对照受试者(P<0.01);(2)Th1细胞因子IL-2水平与Sema 4D mRNA水平负相关(P<0.01),Th2细胞因子IL-4、IL-6和IL-10水平与Sema 4D mRNA水平呈正相关(P<0.01)。结论:Sema4D mRNA在原发免疫性血小板减少症患者中呈高表达,并且分别与Th1和Th2细胞因子水平呈负相关和正相关。Objective：To investigate the Sema 4D genes expressed in primary immune thrombocytopenia and relationship with T helper ceils factor. Methods： Sema 4D mRNA level and Thl/Th2 cytokiues level in peripheral blood mononuclear cells were detec- ted by real-time quantitative polymerase chain reaction and trace sample multi-index streaming protein quantitative technology（CBA） , respectively, the correlation of Sema 4D mRNA level and Th cytokines level was analyzed. Results： （ 1 ） In patients with ITP peripheral blood monocytes Sema 4D volunteers mRNA level was significantly higher than healthy controls （ P 〈 0.01 ）, peripheral blood Thl cyto- kines IL-2 and IFN-3＇ were significantly lower than that of healthy control subjects （P 〈 0.05 or P 〈 0.01 ） , the Th2 cytokines IL-4, IL-6 and IL-10 were significantly higher than that of healthy control subjects （P 〈0.01 ） ; （2） Thl cytokines IL-2 level and Sema 4D mRNA level were negative correlated （ P 〈 0.01 ） , Th2 cytokines IL-4, IL-6 and IL-10 level and Sema 4D mRNA levels were positively correlated （ P 〈 0. O1 ）. Conclusion ： Sema 4D mRNA in primary immune thrombocytopenia in patients with a high expression, and with negative correlation and positive correlation to the level of Thl and Th2 cytokines ,respectively.

关 键 词：原发免疫性血小板减少症 SEMA 4D T辅助细胞因子 

分 类 号：R392[医药卫生—免疫学] R558.2[医药卫生—基础医学]