牛大力多糖的分离纯化及抗氧化活性研究  

Purified and Antioxidant Activity of Water-soluble Polysaccharide from Millettia Speciosa Champ

作　　者：陈蓉蓉[1] 蒲含林[1] 姜华[1] 郑元升[1] 

机构地区：[1]暨南大学细胞生物学系,广东广州510632

出　　处：《食品研究与开发》2014年第3期31-34,共4页Food Research and Development

摘　　要：建立了热水提取、乙醇沉淀、三氟乙酸除蛋白、Sephadex G-75凝胶过滤层析法,从牛大力分离纯化得到一种水溶性多糖,命名为MSP-1。通过红外光谱和离子色谱对其结构和单糖组成进行初步分析,结果表明MSP-1主要由鼠李糖、半乳糖、葡萄糖、甘露糖和果糖组成。同时对牛大力水提物、醇沉物和粗多糖进行体外抗氧化活性研究,结果表明,三者的抗脂质过氧化作用和对·OH自由基和DPPH·自由基清除能力:VC>牛大力水提物>牛大力醇沉物>牛大力粗多糖,并呈量效关系。A water-soluble polysaccharide （MSP-1） was obtained from Millettia Speciosa Champ. by hot water extraction, ethanol precipitation, trifluoroacetic acid method removing proteins and gel permeation chromatography. The structure of MSP-1 was characterized by Ion Chromatography and FTIR spectroscopy. The results showed that MSP-1 was mainly composed of rhamnose, galactose, glucose, mannose and fructose. Additionally, Antioxidant activity in vitro of different extractions from Millettia Speciosa Champ were studied by testing the scavenging effects of ＆#183;OH, DPPH＆#183; and lipid peroxidantion. The results showed that three parts can effectively remove ＆#183;OH, DPPH＆#183;, lipid peroxidantion and was dose-effect relationship. The order of radical scavenging activity was as follow：water extraction〉ethanol precipitation〉crude polysaccharide.

关 键 词：牛大力 多糖 抗氧化 

分 类 号：R284.1[医药卫生—中药学] O657[医药卫生—中医学]