高效液相色谱法测定石见穿中迷迭香酸的含量  

Determination of rosmarinic acid in Salvia chinensis benth. by HPLC

作　　者：高俊峰[1] 丁玲[1] 张鹏[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中华中医药杂志》2014年第4期1192-1193,共2页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国际科技合作专项(No.Z121107002412021)~~

摘　　要：目的:建立石见穿中迷迭香酸的含量测定方法。方法:采用高效液相色谱法,色谱柱为Thermo ODS-2 YHPERSIL(4.6mm×150mm,5μm),流动相为乙腈-0.1%甲酸水(19∶81),流速为1.0mL/min,检测波长为330nm,柱温23℃。结果:迷迭香酸进样量在0.065-1.290μg范围内与峰面积呈良好线性关系,r=0.9999,平均回收率为99.88%,RSD=1.37%(n=6)。结论:本方法简便易行,准确可靠,重复性好,可用于石见穿药材的质量控制。Objective： To establish the content determination method of rosmarinic acid in Salvia chinensis benth.. Methods： Determine the content of rosmarinic acid in Salvia chinensis benth, by HPLC, the chromatographic column was Thermo ODS-2 YHPERSIL （4.6mmxlS0mm, 5μm） , the mobile phase was consisted of acetonitrile-0.1% methanoic acid water （19：81）, the flow rate was 1.0mL/min, the detective wavelength was 330nm and the column temperature was 23 ℃. Results： According to the HPLC determination results, rosmarinic acid showed a good linear relationship in the range of 0.065-1.290pg （r=-0.9999）, with average recovery 99.88% and RSD=l.37% （n=6）. Conclusion： The method was simple, accurate, repeatable and can be used as a quality control method for Salvia chinensis benth..

关 键 词：石见穿 迷迭香酸 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]