取舍怀牛膝通塞脉制剂对PC12细胞损伤保护作用的影响  

Neuroprotective effect of Tongsaimai prescription combined with or without Achyranthes root on PC12 cells injury

作　　者：毕肖林[1,2,3] 狄留庆[1,2,3] 赵晓莉[1,2,3] 

机构地区：[1]南京中医药大学,南京210023 [2]江苏省中药高效给药系统工程技术研究中心,南京210023 [3]南京市中药微丸产业化工程技术研究中心,南京210023

出　　处：《中华中医药杂志》2014年第4期1258-1260,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省优势学科建设工程资助项目(No.ysxk-2010);江苏省"青蓝工程"科技创新团队支持计划[No.(2008)30号];国家自然科学基金项目(No.30873401;No.81073071)~~

摘　　要：目的:观察取舍怀牛膝的通塞脉制剂对不同因素所致的PC12细胞损伤的保护作用。方法:体外培养PC12细胞,建立谷氨酸、过氧化氢、连二亚硫酸钠和氯化钾损伤模型,以MTT法观察取舍怀牛膝通塞脉制剂对PC12细胞存活率的影响。结果:与模型组比较,取舍怀牛膝的通塞脉制剂对各因素所致的PC12细胞损伤均可提高细胞存活率(P<0.05,P<0.01,P<0.001)。结论:取舍怀牛膝的通塞脉制剂对各因素所致的PC12细胞损伤均表现出一定的保护作用。Objective： To compare the protective effects of Tongsaimai prescription combined with or without Achyranthes root on PCI2 cells injury. Methods： Cells were treated with series concentrations of Tongsaimai prescription combined with or without Achyranthes root, then were injured with H202, glutamic acid, Na2S2O4, KC1 individually. The viability of PC12 cells was determined by MTT assay. Results： Compared with model group, Tongsaimai prescription combined with or without Achyranthes root both improved the viability of PC12 ceils （P〈0.05, P〈0.01, P〈0.001）. Conclusion： Tongsaimai prescription combined with or without Achyranthes root both showed the potent neuroprotective effects on PC 12 cells injury.

关 键 词：通塞脉制剂 PC12细胞 缺血性脑损伤 

分 类 号：R285[医药卫生—中药学]