2012年甘肃省艾滋病检测实验室梅毒血清学检测能力验证结果分析  

Analysis of Detection Ability Test Results on Syphilis AIDS Testing Laboratories in Gansu in 2012

作　　者：郁华[1] 许亚宁[1] 白莉[1] 石林[1] 王炳涛[1] 张林才[1] 

机构地区：[1]甘肃省疾病预防控制中心,兰州730000

出　　处：《中国初级卫生保健》2014年第4期71-73,共3页Chinese Primary Health Care

摘　　要：目的通过全省艾滋病检测确证、筛查实验室每年一次梅毒血清学能力验证,不断提高各实验室的梅毒血清学检测水平。方法制定全省统一的能力验证方案,发放制备样品,在规定的时间开展检测,使用Excel软件建设数据库并以使用非梅毒螺旋体和梅毒螺旋体检测试剂为依据,分组进行统计分析。结果本次梅毒血清学检测能力验证活动参评单位是225个实验室,有224个实验室取得合格的成绩,通过了能力验证,1个实验室未取得合格的成绩未通过能力验证;梅毒特异性检测符合率占99.56%;梅毒非特异性检测符合率定性占100%,定量占35.95%。各实验室使用的检测试剂质量基本相同。结论通过开展梅毒血清学检测能力验证活动,可帮助实验人员尽可能发现常规检测中存在的问题,提高梅毒血清学检测水平,保证常规检测工作的质量。OBJECTIVE To increase the level of serological detection of syphilis by laboratory proficiency testing which be applied one time annual among the HIV/AIDS Confirmatory Laboratory and HIV/AIDS Screening Laboratory in Gansu Province. METHODS Establish unified programme of proficiency testing, distribute the serum samples to HIV/AIDS Confirmatory Laboratory and HIV/AIDS Screening Laboratory, then develop detection in the required length of time. Excel was applied to establish the database. RESULTS There were 225 laboratories participated the proficiency test, 224 passed the examination of laboratory proficiency testing, while 1 laboratory failed. The coincidence rate of specificity testing of syphilis was 99.56%. The coincidence rate of nonspecific qualitative test was 100%, qualitative test was 35.95%. CONCLUSION The level of the serological detection of syphilis and the quality of test were increased by laboratory proficiency testing, and this is a way to help the staff of labor to find and solve the problem.

关 键 词：实验室 梅毒血清学检测 甘肃 

分 类 号：R512.91[医药卫生—内科学]