五种多环芳烃化合物对鲤鱼肝微粒体芳烃羟化酶的诱导  

The induction of aryl hydrocarbon hydroxylase(AHH) of carp liver microsome by five aromatic hydrocarbon compounds

作　　者：徐楠[1] 王春霞[1] 呼世斌[2] 徐盈[3] 

机构地区：[1]中国科学院生态环境研究中心环境水化学国家重点实验室,北京100085 [2]西北农业大学基础科学系,陕西杨凌712100 [3]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072

出　　处：《中国环境监测》2000年第6期8-11,共4页Environmental Monitoring in China

基　　金：国家自然科学基金!( 39770 1 52 )

摘　　要：以鲤鱼肝微粒体为实验体系 ,研究了被五种多环芳烃 ( PAH)化合物菲、9,1 0 -菲醌、芘、1 -羟基芘、苯并芘污染后 ,其芳烃羟化酶 ( AHH)的活性变化 ,发现多环芳烃对 AHH的诱导具有良好的剂量效应关系。 AHH活性升高的大小可以作为监测多环芳烃对水体污染程度的一种生物指标。五种多环芳烃对 AHH诱导能力的大小为 1 -羟基芘 >苯并芘 ;9,1 0 -菲醌 >芘 >菲 ,与其毒性大小相关。The aryl hydrocarbon hydroxylase(AHH) activity of carp liver microsome induced by five polyaromatic hydrocarbon (PAH) compounds was examined Five tested PAH named as phenanthrene; 9,10 phenananthrenquinone, pyrene, benzo[a]pyrene, 1 hydroxypyrene induced AHH activity to different extent There existed a good dose effect correlation between the AHH induction and aromatic hydrocarbons concentration The AHH activity of fish liver microsome can be used as a biomarker to evaluate the aromatic hydrocarbon contamination in waters The intense of AHH activity induced by five aromatic hydrocarbons which was related to the toxicity chemicals was as following: 1 hydroxypyrene > benzo[a]pyrene; 9,10 phenananthrenquinone > pyrene > phenanthrene

关 键 词：芳烃羟化酶 多环芳烃 鲤鱼 水体污染 AHH诱导 

分 类 号：X520.322.5[环境科学与工程—环境工程] X132